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- Q5038071 subject Q8462592.
- Q5038071 subject Q8462599.
- Q5038071 subject Q8823787.
- Q5038071 subject Q8906299.
- Q5038071 subject Q8949762.
- Q5038071 abstract "Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is a cell permeable dye generally used in animal cell proliferation research. High concentrations of the dye are toxic to animal cells, however concentrations in the region of 10 micromolar are typically sufficient to give strong staining with minimal cell death. CFDA-SE enters cells by diffusion and is cleaved by intracellular esterase enzymes to form an amine-reactive product, CFSE. This product produces a detectable fluorescence and covalently binds to intracellular lysine residues and other amine sources. Most cell types will excrete a proportion of the dye over the course of the first 24–48 hours following staining. Dye levels should thereafter remain relatively stable in non-dividing cells. If a stained cell divides, the dye is divided equally between the two daughter cells, resulting in both new cells having a CFDA-SE concentration approximately 50% that of the mother cell. A cell stained with CFDA-SE can be kept in culture for several days and fluorescence is detectable in cells following up to 8 successive cell divisions. Fluorescence is typically detected using a flow cytometer on the FL1 detector, with each resulting fluorescent peak representing another round of cell division. The area of each peak is representative of the number of cells in a given division cycle. The staining works best with relatively homogeneous cell populations.CFDA-SE is often erroneously referred to as CFSE, even in scientific literature.".
- Q5038071 thumbnail Carboxyfluorescein_diacetate_succinimidyl_ester.png?width=300.
- Q5038071 wikiPageWikiLink Q1141429.
- Q5038071 wikiPageWikiLink Q127920.
- Q5038071 wikiPageWikiLink Q167198.
- Q5038071 wikiPageWikiLink Q178430.
- Q5038071 wikiPageWikiLink Q189082.
- Q5038071 wikiPageWikiLink Q191807.
- Q5038071 wikiPageWikiLink Q2332446.
- Q5038071 wikiPageWikiLink Q245486.
- Q5038071 wikiPageWikiLink Q3686031.
- Q5038071 wikiPageWikiLink Q3824358.
- Q5038071 wikiPageWikiLink Q4035612.
- Q5038071 wikiPageWikiLink Q418750.
- Q5038071 wikiPageWikiLink Q7868.
- Q5038071 wikiPageWikiLink Q8462592.
- Q5038071 wikiPageWikiLink Q8462599.
- Q5038071 wikiPageWikiLink Q8823787.
- Q5038071 wikiPageWikiLink Q8906299.
- Q5038071 wikiPageWikiLink Q8949762.
- Q5038071 name "6".
- Q5038071 type ChemicalCompound.
- Q5038071 type ChemicalSubstance.
- Q5038071 type ChemicalObject.
- Q5038071 type Thing.
- Q5038071 type Q11173.
- Q5038071 comment "Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is a cell permeable dye generally used in animal cell proliferation research. High concentrations of the dye are toxic to animal cells, however concentrations in the region of 10 micromolar are typically sufficient to give strong staining with minimal cell death. CFDA-SE enters cells by diffusion and is cleaved by intracellular esterase enzymes to form an amine-reactive product, CFSE.".
- Q5038071 label "Carboxyfluorescein diacetate succinimidyl ester".
- Q5038071 depiction Carboxyfluorescein_diacetate_succinimidyl_ester.png.
- Q5038071 name "6-Carboxyfluorescein diacetate succinimidyl ester".