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- Q2124342 subject Q4925926.
- Q2124342 subject Q4926419.
- Q2124342 subject Q7066600.
- Q2124342 abstract "In the field of reverse genetics RMCE (recombinase-mediated cassette exchange) is of increasing relevance. Based on the features of site-specific recombination processes (SSRs), the procedure permits the systematic, repeated modification of higher eukaryotic genomes by targeted integration. For RMCE, this is achieved by the clean exchange of a preexisting gene cassette for an analogous cassette carrying the "gene of interest" (GOI).The genetic modification of mammalian cells is a standard procedure for the production of correctly modified proteins with pharmaceutical relevance. To be successful, the transfer and expression of the transgene has to be highly efficient and should have a largely predictable outcome. Current developments in the field of gene therapy are based on the same principles. Traditional procedures used for transfer of GOIs are not sufficiently reliable, mostly because the relevant epigenetic influences have not been sufficiently explored: transgenes integrate into chromosomes with low efficiency and at loci that provide only sub-optimal conditions for their expression. As a consequence the newly introduced information may not be realized (expressed), the gene(s) may be lost and/or re-insert and they may render the target cells in unstable state. It is exactly this point where RMCE enters the field. The procedure was introduced in 1994 and it uses the tools yeasts and bacteriophages have evolved for the efficient replication of important genetic information:".
- Q2124342 thumbnail RMCE2.PNG?width=300.
- Q2124342 wikiPageExternalLink 111130115822.htm.
- Q2124342 wikiPageWikiLink Q1070877.
- Q2124342 wikiPageWikiLink Q1471601.
- Q2124342 wikiPageWikiLink Q15984992.
- Q2124342 wikiPageWikiLink Q165028.
- Q2124342 wikiPageWikiLink Q178827.
- Q2124342 wikiPageWikiLink Q1897151.
- Q2124342 wikiPageWikiLink Q211675.
- Q2124342 wikiPageWikiLink Q213901.
- Q2124342 wikiPageWikiLink Q218447.
- Q2124342 wikiPageWikiLink Q244145.
- Q2124342 wikiPageWikiLink Q26939.
- Q2124342 wikiPageWikiLink Q26972.
- Q2124342 wikiPageWikiLink Q430117.
- Q2124342 wikiPageWikiLink Q48196.
- Q2124342 wikiPageWikiLink Q4925926.
- Q2124342 wikiPageWikiLink Q4926419.
- Q2124342 wikiPageWikiLink Q4994856.
- Q2124342 wikiPageWikiLink Q5426559.
- Q2124342 wikiPageWikiLink Q656732.
- Q2124342 wikiPageWikiLink Q7066600.
- Q2124342 wikiPageWikiLink Q7531735.
- Q2124342 wikiPageWikiLink Q7531737.
- Q2124342 wikiPageWikiLink Q908966.
- Q2124342 wikiPageWikiLink Q913367.
- Q2124342 comment "In the field of reverse genetics RMCE (recombinase-mediated cassette exchange) is of increasing relevance. Based on the features of site-specific recombination processes (SSRs), the procedure permits the systematic, repeated modification of higher eukaryotic genomes by targeted integration.".
- Q2124342 label "Recombinase-mediated cassette exchange".
- Q2124342 depiction RMCE2.PNG.