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- Q1384835 subject Q7029000.
- Q1384835 subject Q7216294.
- Q1384835 subject Q7217197.
- Q1384835 abstract "In genetics, an expressed sequence tag or EST is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene transcripts, and are instrumental in gene discovery and in gene-sequence determination. The identification of ESTs has proceeded rapidly, with approximately 74.2 million ESTs now available in public databases (e.g. GenBank 1 January 2013, all species).An EST results from one-short sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. They may be represented in databases as either cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand.One can map ESTs to specific chromosome locations using physical mapping techniques, such as radiation hybrid mapping, Happy mapping, or FISH. Alternatively, if the genome of the organism that originated the EST has been sequenced, one can align the EST sequence to that genome using a computer.The current understanding of the human set of genes (as of 2006) includes the existence of thousands of genes based solely on EST evidence. In this respect, ESTs have become a tool to refine the predicted transcripts for those genes, which leads to the prediction of their protein products and ultimately of their function. Moreover, the situation in which those ESTs are obtained (tissue, organ, disease state - e.g. cancer) gives information on the conditions in which the corresponding gene is acting. ESTs contain enough information to permit the design of precise probes for DNA microarrays that then can be used to determine the gene expression.Some authors use the term "EST" to describe genes for which little or no further information exists besides the tag.Nagaraj et al. (2007) have reviewed the significance of ESTs, their properties, methods to analyze EST datasets and their applications in various areas of biology.".
- Q1384835 wikiPageExternalLink est.
- Q1384835 wikiPageExternalLink est.html.
- Q1384835 wikiPageExternalLink EST.
- Q1384835 wikiPageExternalLink tissueinfo.
- Q1384835 wikiPageExternalLink www.estinformatics.org.
- Q1384835 wikiPageExternalLink A858.
- Q1384835 wikiPageExternalLink dbEST_summary.html.
- Q1384835 wikiPageWikiLink Q1128751.
- Q1384835 wikiPageWikiLink Q12078.
- Q1384835 wikiPageWikiLink Q1336182.
- Q1384835 wikiPageWikiLink Q17141848.
- Q1384835 wikiPageWikiLink Q1764062.
- Q1384835 wikiPageWikiLink Q177900.
- Q1384835 wikiPageWikiLink Q2068526.
- Q1384835 wikiPageWikiLink Q26972.
- Q1384835 wikiPageWikiLink Q283478.
- Q1384835 wikiPageWikiLink Q28745.
- Q1384835 wikiPageWikiLink Q591745.
- Q1384835 wikiPageWikiLink Q5971926.
- Q1384835 wikiPageWikiLink Q7029000.
- Q1384835 wikiPageWikiLink Q715789.
- Q1384835 wikiPageWikiLink Q7162.
- Q1384835 wikiPageWikiLink Q720988.
- Q1384835 wikiPageWikiLink Q7216294.
- Q1384835 wikiPageWikiLink Q7217197.
- Q1384835 wikiPageWikiLink Q7280351.
- Q1384835 wikiPageWikiLink Q869146.
- Q1384835 wikiPageWikiLink Q901755.
- Q1384835 comment "In genetics, an expressed sequence tag or EST is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene transcripts, and are instrumental in gene discovery and in gene-sequence determination. The identification of ESTs has proceeded rapidly, with approximately 74.2 million ESTs now available in public databases (e.g. GenBank 1 January 2013, all species).An EST results from one-short sequencing of a cloned cDNA.".
- Q1384835 label "Expressed sequence tag".