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- Q1147272 subject Q7145412.
- Q1147272 subject Q8266666.
- Q1147272 abstract "A cytoplasmic hybrid (or cybrid, a portmanteau of the two words) is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast. Cytoplasts are enucleated cells. This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the fusion. Rho-zero cells are cells which have been depleted of their own mitochondrial DNA by prolonged incubation with ethidium bromide, a chemical which inhibits mitochondrial DNA replication. The rho-zero cells do retain mitochondria and can grow in rich culture medium with certain supplements. They do retain their own nuclear genome. A cybrid is then a hybrid cell which mixes the nuclear genes from one cell with the mitochondrial genes from another cell. Using this powerful tool, it makes it possible to dissociate contribution from the mitochondrial genes vs that of the nuclear genes.Cybrids are valuable in mitochondrial research and have been used to provide suggestive evidence of mitochondrial involvement in Alzheimer's disease, Parkinson's disease, and other conditions.".
- Q1147272 wikiPageExternalLink 7391975.stm.
- Q1147272 wikiPageExternalLink s10691-009-9129-2.
- Q1147272 wikiPageExternalLink WRAP_Kettell_0484023-pais-230210-did_secularism_win_out.pdf.
- Q1147272 wikiPageExternalLink contents.
- Q1147272 wikiPageExternalLink enacted.
- Q1147272 wikiPageExternalLink contents.
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- Q1147272 wikiPageWikiLink Q5380862.
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- Q1147272 wikiPageWikiLink Q7145412.
- Q1147272 wikiPageWikiLink Q7868.
- Q1147272 wikiPageWikiLink Q79899.
- Q1147272 wikiPageWikiLink Q8266666.
- Q1147272 comment "A cytoplasmic hybrid (or cybrid, a portmanteau of the two words) is a eukaryotic cell line produced by the fusion of a whole cell with a cytoplast. Cytoplasts are enucleated cells. This enucleation can be effected by simultaneous application of centrifugal force and treatment of the cell with an agent that disrupts the cytoskeleton. A special case of cybrid formation involves the use of rho-zero cells as the whole cell partner in the fusion.".
- Q1147272 label "Cytoplasmic hybrid".