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Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Transposon_sequencing> ?p ?o }

• Transposon_sequencing abstract "Transposon sequencing (Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) of the transposon insertion sites to identify genes contributing to a function of interest in bacteria.Transposons are highly regulated, discrete DNA segments that can relocate within the genome. They are universal and are found in Eubacteria, Archaea, and Eukarya, including humans. Transposons have a large influence on gene expression and can be used to determine gene function. In fact, when a transposon inserts itself in a gene, the gene's function will be disrupted. Because of that property, transposons have been manipulated for use in insertional mutagenesis. The development of microbial genome sequencing was a major advance for the use of transposon mutagenesis. The function affected by a transposon insertion could be linked to the disrupted gene by sequencing the genome to locate the transposon insertion site. Massively parallel sequencing allows simultaneous sequencing of transposon insertion sites in large mixtures of different mutants. Therefore, genome-wide analysis is feasible if transposons are positioned throughout the genome in a mutant collection.Transposon sequencing requires the creation of a transposon insertion library, which will contain a group of mutants that collectively have transposon insertions in all non-essential genes. The library is grown under the condition that is of interest. Mutants with transposons inserted in genes required for growth under the test condition will diminish in frequency from the population. To identify genes being lost, sequences encompassing the transposon ends are amplified by PCR and sequenced by MPS to determine the location and abundance of each insertion mutation. The importance of each gene for growth under the test condition is determined by comparing the abundance of each mutant before and after growth under the condition being examined. Tn-seq is useful for both the study of a single gene's fitness as well as gene interactions Signature–tagged mutagenesis (STM) is an older technique that also involves pooling transposon insertion mutants to determine the importance of the disrupted genes under selective growth conditions. High-throughput versions of STM use genomic microarrays, which are less accurate and have a lower dynamic range than massively-parallel sequencing. With the invention of next generation sequencing, genomic data became increasingly available. However, despite the increase in genomic data, our knowledge of gene function remains the limiting factor in our understanding of the role genes play. Therefore, a need for a high throughput approach to study genotype-phenotype relationships like Tn-seq was necessary.".
• Transposon_sequencing thumbnail Transposon_insertion_in_Tn-seq.png?width=300.
• Transposon_sequencing wikiPageID "42411667".
• Transposon_sequencing wikiPageLength "11327".
• Transposon_sequencing wikiPageOutDegree "19".
• Transposon_sequencing wikiPageRevisionID "705695863".
• Transposon_sequencing wikiPageWikiLink Archaea.
• Transposon_sequencing wikiPageWikiLink Bacteria.
• Transposon_sequencing wikiPageWikiLink Bacteriophage.
• Transposon_sequencing wikiPageWikiLink Category:DNA_sequencing.
• Transposon_sequencing wikiPageWikiLink Category:Mobile_genetic_elements.
• Transposon_sequencing wikiPageWikiLink DNA.
• Transposon_sequencing wikiPageWikiLink Eukaryote.
• Transposon_sequencing wikiPageWikiLink Gene_expression.
• Transposon_sequencing wikiPageWikiLink Himar_I_Mariner_transposon,.
• Transposon_sequencing wikiPageWikiLink Massive_parallel_sequencing.
• Transposon_sequencing wikiPageWikiLink Mycobacterium_tuberculosis.
• Transposon_sequencing wikiPageWikiLink Polymerase_chain_reaction.
• Transposon_sequencing wikiPageWikiLink Streptococcus_pneumoniae.
• Transposon_sequencing wikiPageWikiLink Transposon_mutagenesis.
• Transposon_sequencing wikiPageWikiLink File:Transposon_insertion_in_Tn-seq.png.
• Transposon_sequencing wikiPageWikiLinkText "Transposon sequencing".
• Transposon_sequencing wikiPageUsesTemplate Template:Multiple_issues.
• Transposon_sequencing wikiPageUsesTemplate Template:Orphan.
• Transposon_sequencing wikiPageUsesTemplate Template:Reflist.
• Transposon_sequencing wikiPageUsesTemplate Template:Technical.
• Transposon_sequencing wikiPageUsesTemplate Template:Update.
• Transposon_sequencing subject Category:DNA_sequencing.
• Transposon_sequencing subject Category:Mobile_genetic_elements.
• Transposon_sequencing comment "Transposon sequencing (Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) of the transposon insertion sites to identify genes contributing to a function of interest in bacteria.Transposons are highly regulated, discrete DNA segments that can relocate within the genome. They are universal and are found in Eubacteria, Archaea, and Eukarya, including humans. Transposons have a large influence on gene expression and can be used to determine gene function.".
• Transposon_sequencing label "Transposon sequencing".
• Transposon_sequencing sameAs Q18354052.
• Transposon_sequencing sameAs m.010rhl1k.
• Transposon_sequencing sameAs Q18354052.
• Transposon_sequencing wasDerivedFrom Transposon_sequencing?oldid=705695863.
• Transposon_sequencing depiction Transposon_insertion_in_Tn-seq.png.
• Transposon_sequencing isPrimaryTopicOf Transposon_sequencing.