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- Toeprinting_assay abstract "The toeprinting assay, also known as the primer extension inhibition assay, is a method used in molecular biology that allows you to examine the interactions between messenger RNA and ribosomes or other RNA-binding proteins. It is different from the more commonly used DNA footprinting assay. The toeprinting assay has been utilized to examine the formation of the translation initiation complex. To do a toeprint assay, you need the mRNA of interest, ribosomes, a DNA primer, free nucleotides, and reverse transcriptase, among other reagents. Reverse transcriptase is an enzyme that catalyzes the formation of DNA from RNA; it is used to generate cDNA because RNA is unstable and difficult to study experimentally. Normally, under these conditions, the reverse transcriptase would simply create a complete cDNA copy of the mRNA of interest. However, when the correct reagents and experimental conditions are used, reverse transcriptase will be blocked by any bound ribosomes, resulting in shorter cDNA fragments called toeprints when the results are observed on a sequencing gel. A schematic of a toeprinting assay can be found here: https://www.researchgate.net/figure/41124064_fig2_A-Schematic-of-the-primer-extension-inhibition-toeprint-assay-illustrating-how.".
- Toeprinting_assay wikiPageExternalLink 41124064_fig2_A-Schematic-of-the-primer-extension-inhibition-toeprint-assay-illustrating-how.
- Toeprinting_assay wikiPageID "34581395".
- Toeprinting_assay wikiPageLength "3505".
- Toeprinting_assay wikiPageOutDegree "11".
- Toeprinting_assay wikiPageRevisionID "705179596".
- Toeprinting_assay wikiPageWikiLink Category:Laboratory_techniques.
- Toeprinting_assay wikiPageWikiLink Category:Molecular_biology.
- Toeprinting_assay wikiPageWikiLink Category:Molecular_biology_techniques.
- Toeprinting_assay wikiPageWikiLink Complementary_DNA.
- Toeprinting_assay wikiPageWikiLink DNA_footprinting.
- Toeprinting_assay wikiPageWikiLink Messenger_RNA.
- Toeprinting_assay wikiPageWikiLink Molecular_biology.
- Toeprinting_assay wikiPageWikiLink Primer_(molecular_biology).
- Toeprinting_assay wikiPageWikiLink RNA-binding_protein.
- Toeprinting_assay wikiPageWikiLink Reverse_transcriptase.
- Toeprinting_assay wikiPageWikiLink Ribosome.
- Toeprinting_assay wikiPageWikiLinkText "Toeprinting assay".
- Toeprinting_assay wikiPageUsesTemplate Template:Expert-subject.
- Toeprinting_assay wikiPageUsesTemplate Template:Multiple_issues.
- Toeprinting_assay wikiPageUsesTemplate Template:Orphan.
- Toeprinting_assay wikiPageUsesTemplate Template:Unreferenced.
- Toeprinting_assay subject Category:Laboratory_techniques.
- Toeprinting_assay subject Category:Molecular_biology.
- Toeprinting_assay subject Category:Molecular_biology_techniques.
- Toeprinting_assay hypernym Method.
- Toeprinting_assay type Software.
- Toeprinting_assay type Laboratory.
- Toeprinting_assay type Technique.
- Toeprinting_assay comment "The toeprinting assay, also known as the primer extension inhibition assay, is a method used in molecular biology that allows you to examine the interactions between messenger RNA and ribosomes or other RNA-binding proteins. It is different from the more commonly used DNA footprinting assay. The toeprinting assay has been utilized to examine the formation of the translation initiation complex.".
- Toeprinting_assay label "Toeprinting assay".
- Toeprinting_assay sameAs Q7812938.
- Toeprinting_assay sameAs m.0j279j0.
- Toeprinting_assay sameAs Q7812938.
- Toeprinting_assay wasDerivedFrom Toeprinting_assay?oldid=705179596.
- Toeprinting_assay isPrimaryTopicOf Toeprinting_assay.