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• Systematic_evolution_of_ligands_by_exponential_enrichment abstract "Systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection or in vitro evolution, is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands. Although SELEX has emerged as the most commonly used name for the procedure, some researchers have referred to it as SAAB (selected and amplified binding site) and CASTing (cyclic amplification and selection of targets) SELEX was first introduced in 1990. In 2015 an special issue was published in the Journal of Molecular Evolution in the honor of quarter century of the SELEX discovery.The process begins with the synthesis of a very large oligonucleotide library consisting of randomly generated sequences of fixed length flanked by constant 5' and 3' ends that serve as primers. For a randomly generated region of length n, the number of possible sequences in the library is 4n (n positions with four possibilities (A,T,C,G) at each position). The sequences in the library are exposed to the target ligand - which may be a protein or a small organic compound - and those that do not bind the target are removed, usually by affinity chromatography. The bound sequences are eluted and amplified by PCR to prepare for subsequent rounds of selection in which the stringency of the elution conditions is increased to identify the tightest-binding sequences. An advancement on the original method allows an RNA library to omit the constant primer regions, which can be difficult to remove after the selection process because they stabilize secondary structures that are unstable when formed by the random region alone.The technique has been used to evolve aptamers of extremely high binding affinity to a variety of target ligands, including small molecules such as ATP and adenosine and proteins such as prions and vascular endothelial growth factor (VEGF). Moreover, SELEX has been used to select high-affinity aptamers for complex targets such as tumor cells. Clinical uses of the technique are suggested by aptamers that bind tumor markers and a VEGF-binding aptamer trade-named Macugen has been approved by the FDA for treatment of macular degeneration. Additionally, SELEX has been utilized to obtain highly specific catalytic DNA or DNAzymes. Several metal-specific DNAzymes have been reported including the GR-5 DNAzyme (lead-specific), the CA1-3 DNAzymes (copper-specific), the 39E DNAzyme (uranyl-specific) and the NaA43 DNAzyme (sodium-specific).A caution to consider in this method is that the selection of extremely high, sub-nanomolar binding affinity entities may not in fact improve specificity for the target molecule. Off-target binding to related molecules could have significant clinical effects.The genetic alphabet, and thus possible aptamers, is expanded using unnatural base pairs was applied to SELEX and high affinity DNA aptamers were generated.".
• Systematic_evolution_of_ligands_by_exponential_enrichment thumbnail Selex1.svg?width=300.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageExternalLink aptamer.freebase.com.
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• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageRevisionID "706063137".
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Adenosine.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Adenosine_triphosphate.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Affinity_chromatography.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Anti-thrombin_aptamers.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Aptamer.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Bacterial_one-hybrid_system.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Biomolecular_structure.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Category:Evolution.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Category:Molecular_biology.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Combinatorial_chemistry.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink DNA.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Deoxyribozyme.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Directionality_(molecular_biology).
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Exodeoxyribonuclease.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Journal_of_Molecular_Evolution.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Ligand_(biochemistry).
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Macular_degeneration.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Molecular_biology.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Nano-.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Oligonucleotide.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Pegaptanib.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Polymerase_chain_reaction.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Primer_(molecular_biology).
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Prion.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Protein.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink RNA.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Small_molecule.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Tumor_marker.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink Vascular_endothelial_growth_factor.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLink File:Selex1.svg.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLinkText "SELEX".
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLinkText "Systematic evolution of ligands by exponential enrichment".
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLinkText "in vitro evolution".
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageWikiLinkText "systematic evolution of ligands by exponential enrichment".
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageUsesTemplate Template:Cite_journal.
• Systematic_evolution_of_ligands_by_exponential_enrichment wikiPageUsesTemplate Template:Reflist.
• Systematic_evolution_of_ligands_by_exponential_enrichment subject Category:Evolution.
• Systematic_evolution_of_ligands_by_exponential_enrichment subject Category:Molecular_biology.
• Systematic_evolution_of_ligands_by_exponential_enrichment hypernym Technique.
• Systematic_evolution_of_ligands_by_exponential_enrichment type TopicalConcept.
• Systematic_evolution_of_ligands_by_exponential_enrichment type Redirect.
• Systematic_evolution_of_ligands_by_exponential_enrichment type Theory.
• Systematic_evolution_of_ligands_by_exponential_enrichment comment "Systematic evolution of ligands by exponential enrichment (SELEX), also referred to as in vitro selection or in vitro evolution, is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands.".
• Systematic_evolution_of_ligands_by_exponential_enrichment label "Systematic evolution of ligands by exponential enrichment".
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs Q907101.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs SELEX.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs Évolution_systématique_de_ligands_par_enrichissement_exponentiel.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs SELEX.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs Evolução_Sistemática_de_Ligantes_por_Enriquecimento_Exponencial.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs m.026p0pn.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs Систематическая_эволюция_лигандов_экспоненциальным_обогащением.
• Systematic_evolution_of_ligands_by_exponential_enrichment sameAs Q907101.
• Systematic_evolution_of_ligands_by_exponential_enrichment wasDerivedFrom Systematic_evolution_of_ligands_by_exponential_enrichment?oldid=706063137.
• Systematic_evolution_of_ligands_by_exponential_enrichment depiction Selex1.svg.
• Systematic_evolution_of_ligands_by_exponential_enrichment isPrimaryTopicOf Systematic_evolution_of_ligands_by_exponential_enrichment.