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- Structure-based_combinatorial_protein_engineering abstract "Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries (lineages) of defined composition designed from structural and probabilistic constraints of the encoded proteins. The development of this technique was driven by fundamental questions about protein structure, function, and evolution, although the technique is generally applicable for the creation of engineered proteins with commercially desirable properties. Combinatorial travel through sequence spacetime is the goal of SCOPE.At its inception, SCOPE was developed as a homology-independent recombination technique to enable the creation of multiple crossover libraries from distantly related genes. In this application, an “exon plate tectonics” design strategy was devised to assemble “equivalent” elements of structure (continental plates) with variability in the junctions linking them (fault lines) to explore global protein space. To create the corresponding library of genes, the breeding scheme of Gregor Mendel was adapted into a PCR strategy to selectively cross hybrid genes, a process of iterative inbreeding to create all possible combinations of coding segments with variable linkages. Genetic complementation in temperature-sensitive E. coli was used as the selection system to successfully identify functional hybrid DNA polymerases of minimal architecture with enhanced phenotypes. SCOPE was then used to construct a synthetic enzyme lineage, which was biochemically characterized to recapitulate the evolutionary divergence of two modern day enzymes. The rapid evolvability of chemical diversity in terpene synthases were demonstrated through processes akin to both Darwinian gradualism and saltation: some mutational pathways show steady, additive changes, whereas others show drastic jumps between contrasting product specificities with single mutational steps. Further, a metric was devised to describe the chemical distance of mutational steps to derive a chemical-based phylogeny relating sequence variation to chemical output. These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or distantly related parental sequences to identify functional novelty among the encoded proteins.".
- Structure-based_combinatorial_protein_engineering wikiPageExternalLink wo.jsp?wo=2005118861.
- Structure-based_combinatorial_protein_engineering wikiPageID "22596736".
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- Structure-based_combinatorial_protein_engineering wikiPageOutDegree "38".
- Structure-based_combinatorial_protein_engineering wikiPageRevisionID "705020202".
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Artificial_gene_synthesis.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Category:Protein_engineering.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Complementation_(genetics).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink DNA_polymerase.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Directed_evolution.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Enzyme.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Escherichia_coli.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Expanded_genetic_code.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Fault_(geology).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Genome.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Gradualism.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Gregor_Mendel.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Homology_(biology).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Hybrid_(biology).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Iteration.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Library_(biology).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Lineage_(evolution).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Nucleic_acid_analogue.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Phenotype.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Phylogenetics.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Plate_tectonics.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Polymerase_chain_reaction.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Protein.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Protein_design.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Protein_engineering.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Protein_folding.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Protein_structure.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Proteome.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Proteomics.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Saltation_(biology).
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Spacetime.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Structural_biology.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Synthase.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Synthetic_biology.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLink Terpene.
- Structure-based_combinatorial_protein_engineering wikiPageWikiLinkText "Structure-based combinatorial protein engineering".
- Structure-based_combinatorial_protein_engineering wikiPageUsesTemplate Template:Cite_journal.
- Structure-based_combinatorial_protein_engineering wikiPageUsesTemplate Template:Fact.
- Structure-based_combinatorial_protein_engineering wikiPageUsesTemplate Template:Sections.
- Structure-based_combinatorial_protein_engineering wikiPageUsesTemplate Template:Unreferenced.
- Structure-based_combinatorial_protein_engineering subject Category:Protein_engineering.
- Structure-based_combinatorial_protein_engineering hypernym Technique.
- Structure-based_combinatorial_protein_engineering type TopicalConcept.
- Structure-based_combinatorial_protein_engineering comment "Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries (lineages) of defined composition designed from structural and probabilistic constraints of the encoded proteins. The development of this technique was driven by fundamental questions about protein structure, function, and evolution, although the technique is generally applicable for the creation of engineered proteins with commercially desirable properties.".
- Structure-based_combinatorial_protein_engineering label "Structure-based combinatorial protein engineering".
- Structure-based_combinatorial_protein_engineering sameAs Q7389216.
- Structure-based_combinatorial_protein_engineering sameAs m.05zrcgy.
- Structure-based_combinatorial_protein_engineering sameAs Q7389216.
- Structure-based_combinatorial_protein_engineering wasDerivedFrom Structure-based_combinatorial_protein_engineering?oldid=705020202.
- Structure-based_combinatorial_protein_engineering isPrimaryTopicOf Structure-based_combinatorial_protein_engineering.