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- Shuttle_vector abstract "A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species [1]. Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types. The main advantage of these vectors is they can be manipulated in E. coli, then used in a system which is more difficult or slower to use (e.g. yeast).Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes (e.g. both Saccharomyces cerevisiae and Escherichia coli) or in different species of bacteria (e.g. both E. coli and Rhodococcus erythropolis). There are also adenovirus shuttle vectors, which can propagate in E. coli and mammals. Shuttle vectors are frequently used to quickly make multiple copies of the gene in E. coli (amplification). They can also be used for in vitro experiments and modifications (e.g. mutagenesis, PCR) One of the most common types of shuttle vectors is the yeast shuttle vector [2]. Almost all commonly used S. cerevisiae vectors are shuttle vectors. Yeast shuttle vectors have components that allow for replication and selection in both E. coli cells and yeast cells. The E. coli component of a yeast shuttle vector includes an origin of replication and a selectable marker, e.g. antibiotic resistance, beta lactamase. The yeast component of a yeast shuttle vector includes an autonomously replicating sequence (ARS), a yeast centromere (CEN), and a yeast selectable marker (e.g. URA3, a gene that encodes an enzyme for uracil synthesis, Lodish et al. 2007).".
- Shuttle_vector wikiPageExternalLink 9.html.
- Shuttle_vector wikiPageExternalLink bv.fcgi?rid=mcb.TOC.
- Shuttle_vector wikiPageExternalLink entrez?db=Books&cmd=Search&term=vector%20AND%20mcb%5Bbook%5D&doptcmdl=TOCView.
- Shuttle_vector wikiPageID "10449512".
- Shuttle_vector wikiPageLength "2532".
- Shuttle_vector wikiPageOutDegree "18".
- Shuttle_vector wikiPageRevisionID "685056159".
- Shuttle_vector wikiPageWikiLink Adenoviridae.
- Shuttle_vector wikiPageWikiLink Autonomously_replicating_sequence.
- Shuttle_vector wikiPageWikiLink Beta-lactamase.
- Shuttle_vector wikiPageWikiLink Category:Molecular_biology.
- Shuttle_vector wikiPageWikiLink Centromere.
- Shuttle_vector wikiPageWikiLink Escherichia_coli.
- Shuttle_vector wikiPageWikiLink Eukaryote.
- Shuttle_vector wikiPageWikiLink In_vitro.
- Shuttle_vector wikiPageWikiLink Mutagenesis.
- Shuttle_vector wikiPageWikiLink Origin_of_replication.
- Shuttle_vector wikiPageWikiLink Plasmid.
- Shuttle_vector wikiPageWikiLink Polymerase_chain_reaction.
- Shuttle_vector wikiPageWikiLink Prokaryote.
- Shuttle_vector wikiPageWikiLink Rhodococcus.
- Shuttle_vector wikiPageWikiLink Saccharomyces_cerevisiae.
- Shuttle_vector wikiPageWikiLink Selectable_marker.
- Shuttle_vector wikiPageWikiLink URA3.
- Shuttle_vector wikiPageWikiLink Vector_(molecular_biology).
- Shuttle_vector wikiPageWikiLinkText "Shuttle vector".
- Shuttle_vector wikiPageWikiLinkText "broad-host-range shuttle vector".
- Shuttle_vector wikiPageWikiLinkText "shuttle vector".
- Shuttle_vector subject Category:Molecular_biology.
- Shuttle_vector hypernym Vector.
- Shuttle_vector type Insect.
- Shuttle_vector type Redirect.
- Shuttle_vector comment "A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species [1]. Therefore, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types. The main advantage of these vectors is they can be manipulated in E. coli, then used in a system which is more difficult or slower to use (e.g. yeast).Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes (e.g.".
- Shuttle_vector label "Shuttle vector".
- Shuttle_vector sameAs Q7505713.
- Shuttle_vector sameAs m.02qdgw0.
- Shuttle_vector sameAs Q7505713.
- Shuttle_vector wasDerivedFrom Shuttle_vector?oldid=685056159.
- Shuttle_vector isPrimaryTopicOf Shuttle_vector.