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- Secretion_assay abstract "Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al. in 1995.Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to \"catch\" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place. Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) technology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.".
- Secretion_assay wikiPageExternalLink nm0301_373.html.
- Secretion_assay wikiPageExternalLink GMDProteinSecretionAssay.htm.
- Secretion_assay wikiPageID "3599041".
- Secretion_assay wikiPageLength "2180".
- Secretion_assay wikiPageOutDegree "14".
- Secretion_assay wikiPageRevisionID "696341102".
- Secretion_assay wikiPageWikiLink Antibody.
- Secretion_assay wikiPageWikiLink Category:Analytical_chemistry.
- Secretion_assay wikiPageWikiLink Category:Cell_biology.
- Secretion_assay wikiPageWikiLink Category:Cytokines.
- Secretion_assay wikiPageWikiLink Category:Proteins.
- Secretion_assay wikiPageWikiLink Cell_biology.
- Secretion_assay wikiPageWikiLink Cytokine.
- Secretion_assay wikiPageWikiLink ELISA.
- Secretion_assay wikiPageWikiLink Flow_cytometry.
- Secretion_assay wikiPageWikiLink Fluorophore.
- Secretion_assay wikiPageWikiLink One_Cell_Systems.
- Secretion_assay wikiPageWikiLink Protein.
- Secretion_assay wikiPageWikiLink Secretion.
- Secretion_assay wikiPageWikiLinkText "Cytokine secretion assay".
- Secretion_assay wikiPageWikiLinkText "Secretion assay".
- Secretion_assay wikiPageWikiLinkText "secretion assay".
- Secretion_assay wikiPageUsesTemplate Template:Cell-biology-stub.
- Secretion_assay wikiPageUsesTemplate Template:Cite_journal.
- Secretion_assay wikiPageUsesTemplate Template:Protein_methods.
- Secretion_assay subject Category:Analytical_chemistry.
- Secretion_assay subject Category:Cell_biology.
- Secretion_assay subject Category:Cytokines.
- Secretion_assay subject Category:Proteins.
- Secretion_assay hypernym Process.
- Secretion_assay type Biomolecule.
- Secretion_assay type Election.
- Secretion_assay type Biomolecule.
- Secretion_assay type Nutrient.
- Secretion_assay type Redirect.
- Secretion_assay comment "Secretion assay is a process used in cell biology to identify cells that are secreting a particular protein (usually a cytokine). It was first developed by Manz et al. in 1995.Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to \"catch\" the secreted molecules.".
- Secretion_assay label "Secretion assay".
- Secretion_assay sameAs Q7444440.
- Secretion_assay sameAs m.09p0kh.
- Secretion_assay sameAs Q7444440.
- Secretion_assay wasDerivedFrom Secretion_assay?oldid=696341102.
- Secretion_assay isPrimaryTopicOf Secretion_assay.