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- Ribonuclease_T1 abstract "Ribonuclease T1 (EC 3.1.27.3, guanyloribonuclease, Aspergillus oryzae ribonuclease, RNase N1, RNase N2, ribonuclease N3, ribonuclease U1, ribonuclease F1, ribonuclease Ch, ribonuclease PP1, ribonuclease SA, RNase F1, ribonuclease C2, binase, RNase Sa, guanyl-specific RNase, RNase G, RNase T1, ribonuclease guaninenucleotido-2'-transferase (cyclizing), ribonuclease N3, ribonuclease N1) is a fungal endonuclease that cleaves single-stranded RNA after guanine residues, i.e., on their 3' end; the most commonly studied form of this enzyme is the version found in the mold Aspergillus oryzae. Owing to its specificity for guanine, RNase T1 is often used to digest denatured RNA prior to sequencing. Similar to other ribonucleases such as barnase and RNase A, ribonuclease T1 has been popular for folding studies.Structurally, ribonuclease T1 is a small α+β protein (104 amino acids) with a four-stranded, antiparallel beta sheet covering a long alpha helix (almost five turns). RNase T1 has two disulfide bonds, Cys2-Cys10 and Cys6-Cys103, of which the latter contributes more to its folding stability; complete reduction of both disulfides usually unfolds the protein, although its folding can be rescued with high salt concentrations.RNase T1 also has four prolines, two of which (Pro39 and Pro55) have cis isomers of their X-Pro peptide bonds. Nonnative isomers of these prolines can retard conformational folding dramatically, folding on a characteristic time scale of 7,000 seconds (almost two hours) at 10°C and pH 5.".
- Ribonuclease_T1 ecNumber "3.1.27.3".
- Ribonuclease_T1 thumbnail RNase-T1_pdb_1ygw.jpg?width=300.
- Ribonuclease_T1 wikiPageID "9759366".
- Ribonuclease_T1 wikiPageLength "4682".
- Ribonuclease_T1 wikiPageOutDegree "18".
- Ribonuclease_T1 wikiPageRevisionID "700458645".
- Ribonuclease_T1 wikiPageWikiLink Alpha_helix.
- Ribonuclease_T1 wikiPageWikiLink Amino_acid.
- Ribonuclease_T1 wikiPageWikiLink Aspergillus_oryzae.
- Ribonuclease_T1 wikiPageWikiLink Barnase.
- Ribonuclease_T1 wikiPageWikiLink Beta_sheet.
- Ribonuclease_T1 wikiPageWikiLink Category:Ribonucleases.
- Ribonuclease_T1 wikiPageWikiLink Endonuclease.
- Ribonuclease_T1 wikiPageWikiLink Enzyme.
- Ribonuclease_T1 wikiPageWikiLink Fungus.
- Ribonuclease_T1 wikiPageWikiLink Guanine.
- Ribonuclease_T1 wikiPageWikiLink Isomer.
- Ribonuclease_T1 wikiPageWikiLink Mold.
- Ribonuclease_T1 wikiPageWikiLink Pancreatic_ribonuclease.
- Ribonuclease_T1 wikiPageWikiLink Peptide_bond.
- Ribonuclease_T1 wikiPageWikiLink Proline.
- Ribonuclease_T1 wikiPageWikiLink RNA.
- Ribonuclease_T1 wikiPageWikiLinkText "Ribonuclease T1".
- Ribonuclease_T1 wikiPageWikiLinkText "ribonuclease T1".
- Ribonuclease_T1 caption "Ribonuclease T1 from Aspergillus oryzae.".
- Ribonuclease_T1 casNumber "9026-12-04".
- Ribonuclease_T1 ecNumber "3.1".
- Ribonuclease_T1 ecnumber "3.1".
- Ribonuclease_T1 goCode "46589".
- Ribonuclease_T1 iubmbEcNumber "3".
- Ribonuclease_T1 name "Ribonuclease T1".
- Ribonuclease_T1 pdb "1".
- Ribonuclease_T1 symbol "rntA".
- Ribonuclease_T1 uniprot "P00651".
- Ribonuclease_T1 wikiPageUsesTemplate Template:Distinguish.
- Ribonuclease_T1 wikiPageUsesTemplate Template:EC_number.
- Ribonuclease_T1 wikiPageUsesTemplate Template:Enzyme.
- Ribonuclease_T1 wikiPageUsesTemplate Template:Enzyme-stub.
- Ribonuclease_T1 wikiPageUsesTemplate Template:Esterases.
- Ribonuclease_T1 wikiPageUsesTemplate Template:MeshName.
- Ribonuclease_T1 wikiPageUsesTemplate Template:Protein.
- Ribonuclease_T1 wikiPageUsesTemplate Template:Reflist.
- Ribonuclease_T1 subject Category:Ribonucleases.
- Ribonuclease_T1 hypernym Endonuclease.
- Ribonuclease_T1 type Biomolecule.
- Ribonuclease_T1 type Enzyme.
- Ribonuclease_T1 type Protein.
- Ribonuclease_T1 type Chemical.
- Ribonuclease_T1 type Redirect.
- Ribonuclease_T1 type Thing.
- Ribonuclease_T1 type Q206229.
- Ribonuclease_T1 type Q8047.
- Ribonuclease_T1 comment "Ribonuclease T1 (EC 3.1.27.3, guanyloribonuclease, Aspergillus oryzae ribonuclease, RNase N1, RNase N2, ribonuclease N3, ribonuclease U1, ribonuclease F1, ribonuclease Ch, ribonuclease PP1, ribonuclease SA, RNase F1, ribonuclease C2, binase, RNase Sa, guanyl-specific RNase, RNase G, RNase T1, ribonuclease guaninenucleotido-2'-transferase (cyclizing), ribonuclease N3, ribonuclease N1) is a fungal endonuclease that cleaves single-stranded RNA after guanine residues, i.e., on their 3' end; the most commonly studied form of this enzyme is the version found in the mold Aspergillus oryzae. ".
- Ribonuclease_T1 label "Ribonuclease T1".
- Ribonuclease_T1 differentFrom Ribonuclease_T.
- Ribonuclease_T1 differentFrom Ribonuclease_T2.
- Ribonuclease_T1 sameAs Q4394321.
- Ribonuclease_T1 sameAs Ribonucleasa_T1.
- Ribonuclease_T1 sameAs Ribonucléase_T1.
- Ribonuclease_T1 sameAs m.02prb4x.
- Ribonuclease_T1 sameAs Рибонуклеаза_Т1.
- Ribonuclease_T1 sameAs Ribonukleaza_T1.
- Ribonuclease_T1 sameAs Ribonukleaza_T1.
- Ribonuclease_T1 sameAs Q4394321.
- Ribonuclease_T1 wasDerivedFrom Ribonuclease_T1?oldid=700458645.
- Ribonuclease_T1 depiction RNase-T1_pdb_1ygw.jpg.
- Ribonuclease_T1 isPrimaryTopicOf Ribonuclease_T1.
- Ribonuclease_T1 name "Ribonuclease T1".
