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- Reverse_Transcription_Loop-mediated_Isothermal_Amplification abstract "Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a technique for the amplification of RNA.Within the last 10 years of its development, applications of the LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification have been widely used. This method was then improved by taking it a step further and combining it with a reverse transcription phase to allow for the detection of RNA. RT-LAMP is a one step nucleic acid amplification method that is used to diagnose infectious disease caused by bacteria or viruses. Although it has not been formally recognised by NAT, the method has been developed into many commercial kits that can be used for the identification of pathogens. The commonly used PCR method is able to generate millions of copies of the target strand. This process relies on thermal cycling, cycles of heating and cooling to facilitate the DNA replication. RT-LAMP does not require these cycles and is performed at a constant temperature between 60 and 65 °C. Similar to RT-PCR, RT-LAMP uses reverse transcriptase to synthesize complementary DNA (cDNA) from RNA sequences. This cDNA is then amplified using DNA polymerase, generating 10^9 copies per hour.RT-LAMP is used in the detection of viruses. This method can be very effective in detecting viruses with an RNA genome (Group II, IV, and V based on the Baltimorevirus classification system).".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification thumbnail Initiation_step_of_RT-LAMP_method.png?width=300.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageExternalLink e.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageExternalLink ),.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageID "42447237".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageLength "9343".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageOutDegree "15".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageRevisionID "656271377".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Category:Genetics.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Category:Molecular_biology.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Category:RNA.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Complementary_DNA.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink File:Cycling_amplification_and_elongation_steps_of_RT-LAMP_method.png.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink File:Initiation_step_of_RT-LAMP_method.png.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Loop-mediated_isothermal_amplification.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Nucleic_acid_test.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Polymerase_chain_reaction.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink RNA.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Reverse_transcriptase.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Reverse_transcription_polymerase_chain_reaction.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Thermal_cycler.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Virus.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageWikiLink Virus_classification.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageUsesTemplate Template:Orphan.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wikiPageUsesTemplate Template:Reflist.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification subject Category:Genetics.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification subject Category:Molecular_biology.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification subject Category:RNA.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification hypernym Technique.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification type TopicalConcept.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification comment "Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a technique for the amplification of RNA.Within the last 10 years of its development, applications of the LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification have been widely used. This method was then improved by taking it a step further and combining it with a reverse transcription phase to allow for the detection of RNA.".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification label "Reverse Transcription Loop-mediated Isothermal Amplification".
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification sameAs Q18394182.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification sameAs m.010pr0c7.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification sameAs Q18394182.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification wasDerivedFrom Reverse_Transcription_Loop-mediated_Isothermal_Amplification?oldid=656271377.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification depiction Initiation_step_of_RT-LAMP_method.png.
- Reverse_Transcription_Loop-mediated_Isothermal_Amplification isPrimaryTopicOf Reverse_Transcription_Loop-mediated_Isothermal_Amplification.