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- Restriction_landmark_genomic_scanning abstract "Restriction Landmark Genomic Scanning (or RLGS) is a genome analysis method that allows for rapid simultaneous visualization of thousands of landmarks, or restriction sites. Using a combination of restriction enzymes some of which are specific to DNA modifications, the technique can be used to visualize differences in methylation levels across the genome of a given organism. RLGS employs direct labeling of DNA, which is first cut by a specific series of restriction enzymes, and then labeled by a radioactive isotope (usually phosphorus-32). A two-dimensional electrophoresis process is then employed, yielding high-resolution results. The radioactive second-dimension gel is then allowed to expose a large sheet of film. The radiation produced by the radioactive labeling will cause the film to be exposed wherever the restriction fragments have migrated during electrophoresis. The film is then developed, yielding a visual representation of the results in the form of an autoradiograph. The same combination of restriction enzymes will produce the same pattern of 'spots' from samples from the same organisms, but different patterns for different types of organism. For example, human and mouse DNA will produce distinctly different patterns when treated with the same combination of enzymes. These finished auto-rads can be examined against each other, revealing any changes in gene expression that lead to visual differences in the film. Each autoradiograph contains thousands of spots, each corresponding to a labeled DNA restriction landmark.RLGS becomes very useful when doing whole-genome scans, and can effectively do the work of thousands of polymerase chain reactions at once. It readily detects alterations deviating from normal, and thus is exceptionally effective in identifying hyper/hypomethylation in tumors, deletions or amplifications of genes, or simply changes in gene expression throughout the development of an organism.".
- Restriction_landmark_genomic_scanning wikiPageExternalLink accession_report.cgi?id=MGI:71287.
- Restriction_landmark_genomic_scanning wikiPageExternalLink smiragliaweb2.jpg.
- Restriction_landmark_genomic_scanning wikiPageID "12830500".
- Restriction_landmark_genomic_scanning wikiPageLength "3251".
- Restriction_landmark_genomic_scanning wikiPageOutDegree "24".
- Restriction_landmark_genomic_scanning wikiPageRevisionID "700730390".
- Restriction_landmark_genomic_scanning wikiPageWikiLink Autoradiograph.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Category:Genomics.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Category:Laboratory_techniques.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Category:Molecular_biology.
- Restriction_landmark_genomic_scanning wikiPageWikiLink DNA.
- Restriction_landmark_genomic_scanning wikiPageWikiLink DNA_methylation.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Deletion_(genetics).
- Restriction_landmark_genomic_scanning wikiPageWikiLink Electrophoresis.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Epigenetics.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Gene_expression.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Genome.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Isotope.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Neoplasm.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Phosphorus.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Photographic_film.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Polymerase_chain_reaction.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Radioactive_decay.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Restriction_enzyme.
- Restriction_landmark_genomic_scanning wikiPageWikiLink Two-dimensional_gel_electrophoresis.
- Restriction_landmark_genomic_scanning wikiPageWikiLinkText "Restriction Landmark Genome Scanning (RLGS)".
- Restriction_landmark_genomic_scanning wikiPageWikiLinkText "Restriction landmark genomic scanning".
- Restriction_landmark_genomic_scanning wikiPageWikiLinkText "restriction landmark genomic scanning".
- Restriction_landmark_genomic_scanning wikiPageUsesTemplate Template:Biology-stub.
- Restriction_landmark_genomic_scanning wikiPageUsesTemplate Template:Cite_journal.
- Restriction_landmark_genomic_scanning wikiPageUsesTemplate Template:Reflist.
- Restriction_landmark_genomic_scanning subject Category:Genomics.
- Restriction_landmark_genomic_scanning subject Category:Laboratory_techniques.
- Restriction_landmark_genomic_scanning subject Category:Molecular_biology.
- Restriction_landmark_genomic_scanning hypernym Method.
- Restriction_landmark_genomic_scanning type Software.
- Restriction_landmark_genomic_scanning type Genomic.
- Restriction_landmark_genomic_scanning type Laboratory.
- Restriction_landmark_genomic_scanning type Omic.
- Restriction_landmark_genomic_scanning type Technique.
- Restriction_landmark_genomic_scanning comment "Restriction Landmark Genomic Scanning (or RLGS) is a genome analysis method that allows for rapid simultaneous visualization of thousands of landmarks, or restriction sites. Using a combination of restriction enzymes some of which are specific to DNA modifications, the technique can be used to visualize differences in methylation levels across the genome of a given organism.".
- Restriction_landmark_genomic_scanning label "Restriction landmark genomic scanning".
- Restriction_landmark_genomic_scanning sameAs Q7316319.
- Restriction_landmark_genomic_scanning sameAs m.02x6qp7.
- Restriction_landmark_genomic_scanning sameAs Q7316319.
- Restriction_landmark_genomic_scanning wasDerivedFrom Restriction_landmark_genomic_scanning?oldid=700730390.
- Restriction_landmark_genomic_scanning isPrimaryTopicOf Restriction_landmark_genomic_scanning.