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Recombinant_AAV_mediated_genome_engineering abstract "Recombinant adeno-associated virus (rAAV) based genome engineering is a genome editing platform centered on the use of rAAV vectors that enables insertion, deletion or substitiution of DNA sequences into the genomes of live mammalian cells. The technique builds on Capecchi and Smithies’ Nobel Prize–winning discovery that homologous recombination (HR), a natural hi-fidelity DNA repair mechanism, can be harnessed to perform precise genome alterations in mice. rAAV mediated genome-editing improves the efficiency of this technique to permit genome engineering in any pre-established and differentiated human cell line, which, in contrast to mouse ES cells, have low rates of HR.The technique has been widely adopted for use in engineering human cell lines to generate isogenic human disease models. It has also been used to optimize bioproducer cell lines for the biomanufacturing of protein vaccines and therapeutics. In addition, due to the non-pathogenic nature of rAAV, it has emerged as a desirable vector for performing gene therapy in live patients.".
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Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Adeno-associated_virus.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Biological_engineering.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Category:Biochemistry.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Category:Recombinant_proteins.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink DNA.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Genome_editing.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Genome_engineering.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Homing_endonuclease.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Homologous_recombination.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Horizon_Discovery.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Isogenic_human_disease_models.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Meganuclease.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Nobel_Prize.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Recombinant_DNA.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Single-nucleotide_polymorphism.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLink Zinc_finger_nuclease.
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLinkText "Recombinant AAV mediated genome engineering".
Recombinant_AAV_mediated_genome_engineering wikiPageWikiLinkText "rAAV".
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Recombinant_AAV_mediated_genome_engineering subject Category:Biochemistry.
Recombinant_AAV_mediated_genome_engineering subject Category:Recombinant_proteins.
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Recombinant_AAV_mediated_genome_engineering type Product.
Recombinant_AAV_mediated_genome_engineering comment "Recombinant adeno-associated virus (rAAV) based genome engineering is a genome editing platform centered on the use of rAAV vectors that enables insertion, deletion or substitiution of DNA sequences into the genomes of live mammalian cells. The technique builds on Capecchi and Smithies’ Nobel Prize–winning discovery that homologous recombination (HR), a natural hi-fidelity DNA repair mechanism, can be harnessed to perform precise genome alterations in mice.".
Recombinant_AAV_mediated_genome_engineering label "Recombinant AAV mediated genome engineering".
Recombinant_AAV_mediated_genome_engineering sameAs Q17077643.
Recombinant_AAV_mediated_genome_engineering sameAs m.0gfd14t.
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