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- Rapid_amplification_of_cDNA_ends abstract "Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region. RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. This technique is sometimes called one-sided PCR or anchored PCR.The first step in RACE is to use reverse transcription to produce a cDNA copy of a region of the RNA transcript. In this process, an unknown end portion of a transcript is copied using a known sequence from the center of the transcript. The copied region is bounded by the known sequence, at either the 5' or 3' end. The protocols for 5' or 3' RACES differ slightly. 5' RACE-PCR begins using mRNA as a template for a first round of cDNA synthesis (or reverse transcription) reaction using an anti-sense (reverse) oligonucleotide primer that recognizes a known sequence in the middle of the gene of interest; the primer is called a gene specific primer (GSP). The primer binds to the mRNA, and the enzyme reverse transcriptase adds base pairs to the 3' end of the primer to generate a specific single-stranded cDNA product; this is the reverse complement of the mRNA. Following cDNA synthesis, the enzyme terminal deoxynucleotidyl transferase (TdT) is used to add a string of identical nucleotides, known as a homopolymeric tail, to the 3' end of the cDNA. (There are some other ways to add the 3'-terminal sequence for the first strand of the de novo cDNA synthesis which are much more efficient than homopolymeric tailing, but the sense of the method remains the same). A PCR reaction is then carried out, which uses a second anti-sense gene specific primer (GSP2) that binds to the known sequence, and a sense (forward) universal primer (UP) that binds the homopolymeric tail added to the 3' ends of the cDNAs to amplify a cDNA product from the 5' end.3' RACE-PCR uses the natural polyA tail that exists at the 3' end of all eukaryotic mRNAs for priming during reverse transcription, so this method does not require the addition of nucleotides by TdT. cDNAs are generated using an Oligo-dT-adaptor primer (a primer with a short sequence of deoxy-thymine nucleotides) that complements the polyA stretch and adds a special adaptor sequence to the 5' end of each cDNA. PCR is then used to amplify 3' cDNA from a known region using a sense GSP, and an anti-sense primer complementary to the adaptor sequence.".
- Rapid_amplification_of_cDNA_ends wikiPageID "1323803".
- Rapid_amplification_of_cDNA_ends wikiPageLength "3508".
- Rapid_amplification_of_cDNA_ends wikiPageOutDegree "20".
- Rapid_amplification_of_cDNA_ends wikiPageRevisionID "704108104".
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Category:Genetic_mapping.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Category:Molecular_biology.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Complementary_DNA.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Directionality_(molecular_biology).
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Enzyme.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Molecular_biology.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Nucleotide.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Oligo-dT.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Oligonucleotide.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Polyadenylation.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Polymerase_chain_reaction.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Primer_(molecular_biology).
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink RNA.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Reverse_transcriptase.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Reverse_transcription_polymerase_chain_reaction.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLink Terminal_deoxynucleotidyl_transferase.
- Rapid_amplification_of_cDNA_ends wikiPageWikiLinkText "RACE".
- Rapid_amplification_of_cDNA_ends wikiPageWikiLinkText "Rapid amplification of cDNA ends".
- Rapid_amplification_of_cDNA_ends subject Category:Genetic_mapping.
- Rapid_amplification_of_cDNA_ends subject Category:Molecular_biology.
- Rapid_amplification_of_cDNA_ends hypernym Technique.
- Rapid_amplification_of_cDNA_ends type TopicalConcept.
- Rapid_amplification_of_cDNA_ends type Genomic.
- Rapid_amplification_of_cDNA_ends comment "Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR). The amplified cDNA copies are then sequenced and, if long enough, should map to a unique genomic region.".
- Rapid_amplification_of_cDNA_ends label "Rapid amplification of cDNA ends".
- Rapid_amplification_of_cDNA_ends sameAs Q381434.
- Rapid_amplification_of_cDNA_ends sameAs RACE-PCR.
- Rapid_amplification_of_cDNA_ends sameAs RACE-PCR.
- Rapid_amplification_of_cDNA_ends sameAs RACE.
- Rapid_amplification_of_cDNA_ends sameAs RACE_PCR.
- Rapid_amplification_of_cDNA_ends sameAs m.04sstf.
- Rapid_amplification_of_cDNA_ends sameAs Q381434.
- Rapid_amplification_of_cDNA_ends wasDerivedFrom Rapid_amplification_of_cDNA_ends?oldid=704108104.
- Rapid_amplification_of_cDNA_ends isPrimaryTopicOf Rapid_amplification_of_cDNA_ends.