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- Promoter_bashing abstract "Promoter bashing is a technique used in molecular biology to identify how certain regions of a DNA strand, commonly promoters, affect the transcription of downstream genes. Under normal circumstances, proteins bind to the promoter and activate or repress transcription. In a promoter bashing assay, specific point mutations or deletions are made in specific regions of the promoter and the transcription of the gene is then measured. The contribution of a region of the promoter can be observed by the level of transcription. If a mutation or deletion changes the level of transcription, then it is known that that region of the promoter may be a binding site or other regulatory element.Promoter bashing is often done with deletions from either the 5' or 3' end of the DNA strand; this assay is easier to perform based on repeated restriction digestion and gel-purifying fragments of specific sizes. It is often easiest to ligate the promoter into the reporter, generate a large amount of the reporter construct using PCR or growth in bacteria, and then perform serial restriction digests on this sample. The ability of upstream promoters can be easily assayed by removing segments from the 5' end, and the same for the 3' end of the strand for downstream promoters.As the promoter commonly contains binding sequences for proteins affecting transcription, those proteins are also necessary when testing the effects of the promoter. Proteins which associate with the promoter can be identified using an electrophoretic mobility shift assay (EMSA), and the effects of inclusion or exclusion of the proteins with the mutagenized promoters can be assessed in the assay. This allows the use of promoter bashing to not only discover the location on the DNA strand which affects transcription, but also the proteins which affect that strand. The effects of protein interactions with each other as well as the binding sites can also be assayed in this way; candidate proteins must instead be identified by protein/protein interaction assays instead of an EMSA.".
- Promoter_bashing thumbnail Promoter_bashing.svg?width=300.
- Promoter_bashing wikiPageID "16185953".
- Promoter_bashing wikiPageLength "8116".
- Promoter_bashing wikiPageOutDegree "25".
- Promoter_bashing wikiPageRevisionID "608440070".
- Promoter_bashing wikiPageWikiLink Assay.
- Promoter_bashing wikiPageWikiLink Category:Molecular_biology_techniques.
- Promoter_bashing wikiPageWikiLink Category:Molecular_genetics.
- Promoter_bashing wikiPageWikiLink DNA.
- Promoter_bashing wikiPageWikiLink DNA_footprinting.
- Promoter_bashing wikiPageWikiLink DNA_sequencing.
- Promoter_bashing wikiPageWikiLink Deletion_(genetics).
- Promoter_bashing wikiPageWikiLink Directionality_(molecular_biology).
- Promoter_bashing wikiPageWikiLink Electrophoretic_mobility_shift_assay.
- Promoter_bashing wikiPageWikiLink File:Promoter_bashing.svg.
- Promoter_bashing wikiPageWikiLink Gel_electrophoresis.
- Promoter_bashing wikiPageWikiLink Gene_expression.
- Promoter_bashing wikiPageWikiLink Molecular_biology.
- Promoter_bashing wikiPageWikiLink Molecular_cloning.
- Promoter_bashing wikiPageWikiLink Point_mutation.
- Promoter_bashing wikiPageWikiLink Polymerase_chain_reaction.
- Promoter_bashing wikiPageWikiLink Promoter_(genetics).
- Promoter_bashing wikiPageWikiLink Protein.
- Promoter_bashing wikiPageWikiLink Reporter_gene.
- Promoter_bashing wikiPageWikiLink Restriction_digest.
- Promoter_bashing wikiPageWikiLink Site-directed_mutagenesis.
- Promoter_bashing wikiPageWikiLink Transcription_(genetics).
- Promoter_bashing wikiPageWikiLinkText "Promoter bashing".
- Promoter_bashing wikiPageWikiLinkText "promoter bashing".
- Promoter_bashing wikiPageUsesTemplate Template:Molecular_genetics_methods.
- Promoter_bashing wikiPageUsesTemplate Template:Reflist.
- Promoter_bashing subject Category:Molecular_biology_techniques.
- Promoter_bashing subject Category:Molecular_genetics.
- Promoter_bashing hypernym Technique.
- Promoter_bashing type TopicalConcept.
- Promoter_bashing type Page.
- Promoter_bashing type Technique.
- Promoter_bashing comment "Promoter bashing is a technique used in molecular biology to identify how certain regions of a DNA strand, commonly promoters, affect the transcription of downstream genes. Under normal circumstances, proteins bind to the promoter and activate or repress transcription. In a promoter bashing assay, specific point mutations or deletions are made in specific regions of the promoter and the transcription of the gene is then measured.".
- Promoter_bashing label "Promoter bashing".
- Promoter_bashing sameAs Q17092563.
- Promoter_bashing sameAs m.03wc8gl.
- Promoter_bashing sameAs Q17092563.
- Promoter_bashing wasDerivedFrom Promoter_bashing?oldid=608440070.
- Promoter_bashing depiction Promoter_bashing.svg.
- Promoter_bashing isPrimaryTopicOf Promoter_bashing.