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- Polyhistidine-tag abstract "A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag (registered by EMD Biosciences). The tag was invented by Roche, although the use of histidines and its vectors are distributed by Qiagen. Various purification kits for histidine-tagged proteins are available from Qiagen, Sigma, Thermo Scientific, GE Healthcare, Macherey-Nagel, Clontech, Bio-Rad, and others.The use of the tag for academic users is unrestricted; however, commercial users must pay royalties to Roche. The original patent expired on 11 Feb 2003, and thus should be now public property; current claims to royalties are based on a much narrower set of more recent patents. Suitable tag sequences are available free for commercial use; for example, MK(HQ)6 may be used for enhanced expression in E. coli and tag removal. The total number of histidine residues may vary in the tag. The his-tag may also be followed by a suitable amino acid sequence that facilitates a removal of the polyhistidine-tag using endopeptidases. This extra sequence is not necessary if exopeptidases are used to remove N-terminal His-tags (e.g., Qiagen TAGZyme). Furthermore, exopeptidase cleavage may solve the unspecific cleavage observed when using endoprotease-based tag removal. Polyhistidine-tags are often used for affinity purification of genetically modified proteins.".
- Polyhistidine-tag thumbnail Simple_column_for_Ni2+-affinity_chromatography.jpg?width=300.
- Polyhistidine-tag wikiPageExternalLink Cobalt-based-Protein-Purification-Resin-Does-Not-Bind-E--coli-SlyD,-A-Common-Contaminant-in-Ni-NTA-IMAC.
- Polyhistidine-tag wikiPageID "450993".
- Polyhistidine-tag wikiPageLength "13452".
- Polyhistidine-tag wikiPageOutDegree "64".
- Polyhistidine-tag wikiPageRevisionID "695657408".
- Polyhistidine-tag wikiPageWikiLink Affinity_chromatography.
- Polyhistidine-tag wikiPageWikiLink Amino_acid.
- Polyhistidine-tag wikiPageWikiLink Antibody.
- Polyhistidine-tag wikiPageWikiLink Bio-Rad_Laboratories.
- Polyhistidine-tag wikiPageWikiLink C-terminus.
- Polyhistidine-tag wikiPageWikiLink Category:Biochemistry_methods.
- Polyhistidine-tag wikiPageWikiLink Category:Molecular_biology.
- Polyhistidine-tag wikiPageWikiLink Category:Protein_structure.
- Polyhistidine-tag wikiPageWikiLink Cell_(biology).
- Polyhistidine-tag wikiPageWikiLink Centrifugation.
- Polyhistidine-tag wikiPageWikiLink Chelation.
- Polyhistidine-tag wikiPageWikiLink Cobalt.
- Polyhistidine-tag wikiPageWikiLink Conformational_change.
- Polyhistidine-tag wikiPageWikiLink Divalent.
- Polyhistidine-tag wikiPageWikiLink Dual-polarization_interferometry.
- Polyhistidine-tag wikiPageWikiLink ELISA.
- Polyhistidine-tag wikiPageWikiLink Endopeptidase.
- Polyhistidine-tag wikiPageWikiLink Enzyme.
- Polyhistidine-tag wikiPageWikiLink Escherichia_coli.
- Polyhistidine-tag wikiPageWikiLink Ethylenediaminetetraacetic_acid.
- Polyhistidine-tag wikiPageWikiLink Eukaryote.
- Polyhistidine-tag wikiPageWikiLink Exopeptidase.
- Polyhistidine-tag wikiPageWikiLink File:Simple_column_for_Ni2+-affinity_chromatography.jpg.
- Polyhistidine-tag wikiPageWikiLink GE_Healthcare.
- Polyhistidine-tag wikiPageWikiLink Genetic_code.
- Polyhistidine-tag wikiPageWikiLink Genetic_engineering.
- Polyhistidine-tag wikiPageWikiLink Glutathione_S-transferase.
- Polyhistidine-tag wikiPageWikiLink Histidine.
- Polyhistidine-tag wikiPageWikiLink Imidazole.
- Polyhistidine-tag wikiPageWikiLink Iminodiacetic_acid.
- Polyhistidine-tag wikiPageWikiLink Iron.
- Polyhistidine-tag wikiPageWikiLink Lysozyme.
- Polyhistidine-tag wikiPageWikiLink Merck_Group.
- Polyhistidine-tag wikiPageWikiLink Methionine.
- Polyhistidine-tag wikiPageWikiLink Microtiter_plate.
- Polyhistidine-tag wikiPageWikiLink N-terminus.
- Polyhistidine-tag wikiPageWikiLink Nickel.
- Polyhistidine-tag wikiPageWikiLink Nitrilotriacetic_acid.
- Polyhistidine-tag wikiPageWikiLink Peptide_sequence.
- Polyhistidine-tag wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Polyhistidine-tag wikiPageWikiLink Polymerase_chain_reaction.
- Polyhistidine-tag wikiPageWikiLink Primer_(molecular_biology).
- Polyhistidine-tag wikiPageWikiLink Protein.
- Polyhistidine-tag wikiPageWikiLink Protein_microarray.
- Polyhistidine-tag wikiPageWikiLink Protein_tag.
- Polyhistidine-tag wikiPageWikiLink Qiagen.
- Polyhistidine-tag wikiPageWikiLink Royalty_payment.
- Polyhistidine-tag wikiPageWikiLink Sigma-Aldrich.
- Polyhistidine-tag wikiPageWikiLink Start_codon.
- Polyhistidine-tag wikiPageWikiLink Stop_codon.
- Polyhistidine-tag wikiPageWikiLink Streptavidin.
- Polyhistidine-tag wikiPageWikiLink Tandem_affinity_purification.
- Polyhistidine-tag wikiPageWikiLink Thermo_Fisher_Scientific.
- Polyhistidine-tag wikiPageWikiLink Western_blot.
- Polyhistidine-tag wikiPageWikiLink Yeast.
- Polyhistidine-tag wikiPageWikiLink File:His-tag-primers.png.
- Polyhistidine-tag wikiPageWikiLink File:His-tag.png.
- Polyhistidine-tag wikiPageWikiLink Media:His-tag.png.
- Polyhistidine-tag wikiPageWikiLinkText "6xHis".
- Polyhistidine-tag wikiPageWikiLinkText "His".
- Polyhistidine-tag wikiPageWikiLinkText "His-tagged".
- Polyhistidine-tag wikiPageWikiLinkText "Polyhistidine-tag".
- Polyhistidine-tag wikiPageWikiLinkText "histidine (His) tag".
- Polyhistidine-tag wikiPageWikiLinkText "poly(His) tag".
- Polyhistidine-tag wikiPageWikiLinkText "polyhistidine".
- Polyhistidine-tag wikiPageWikiLinkText "polyhistidine-tag".
- Polyhistidine-tag wikiPageUsesTemplate Template:CN.
- Polyhistidine-tag wikiPageUsesTemplate Template:Protein_methods.
- Polyhistidine-tag subject Category:Biochemistry_methods.
- Polyhistidine-tag subject Category:Molecular_biology.
- Polyhistidine-tag subject Category:Protein_structure.
- Polyhistidine-tag hypernym Motif.
- Polyhistidine-tag type Protein.
- Polyhistidine-tag type Biophysic.
- Polyhistidine-tag type Method.
- Polyhistidine-tag type Technique.
- Polyhistidine-tag comment "A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag (registered by EMD Biosciences). The tag was invented by Roche, although the use of histidines and its vectors are distributed by Qiagen.".
- Polyhistidine-tag label "Polyhistidine-tag".
- Polyhistidine-tag sameAs Q7226501.
- Polyhistidine-tag sameAs Polyhistidin-Tag.
- Polyhistidine-tag sameAs Étiquette_poly-histidine.
- Polyhistidine-tag sameAs Hisタグ.
- Polyhistidine-tag sameAs Etiqueta_de_poli-histidina.
- Polyhistidine-tag sameAs m.02b06n.
- Polyhistidine-tag sameAs Polihistidinski_podaljšek.
- Polyhistidine-tag sameAs Полігістидиновий_теґ.
- Polyhistidine-tag sameAs Q7226501.
- Polyhistidine-tag wasDerivedFrom Polyhistidine-tag?oldid=695657408.
- Polyhistidine-tag depiction Simple_column_for_Ni2+-affinity_chromatography.jpg.
- Polyhistidine-tag isPrimaryTopicOf Polyhistidine-tag.