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- Phenol–chloroform_extraction abstract "Phenol–chloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for purifying nucleic acids and eliminating proteins. In brief, aqueous samples are mixed with equal volumes of a phenol:chloroform mixture. After mixing, the mixture is centrifuged and two distinct phases are formed, because the phenol:chloroform mixture is immiscible with water. The aqueous phase is on top because it is less dense than the organic phase (phenol:chloroform). The proteins will partition into the lower organic phase while the nucleic acids (as well as other contaminants such as salts, sugars, etc.) remain in the upper aqueous phase. The upper aqueous phase is pipetted off and care is taken to avoid pipetting any of the organic phase or material at the interface. This procedure is often performed multiple times to increase the purity of the DNA.If the mixture is acidic, DNA will precipitate into the organic phase while RNA remains in the aqueous phase due to DNA being more readily neutralised than RNA.".
- Phenol–chloroform_extraction wikiPageID "41047668".
- Phenol–chloroform_extraction wikiPageLength "1284".
- Phenol–chloroform_extraction wikiPageOutDegree "14".
- Phenol–chloroform_extraction wikiPageRevisionID "693943068".
- Phenol–chloroform_extraction wikiPageWikiLink Biochemistry.
- Phenol–chloroform_extraction wikiPageWikiLink Category:Biochemistry_methods.
- Phenol–chloroform_extraction wikiPageWikiLink Category:Molecular_biology.
- Phenol–chloroform_extraction wikiPageWikiLink Chloroform.
- Phenol–chloroform_extraction wikiPageWikiLink DNA.
- Phenol–chloroform_extraction wikiPageWikiLink Interface_(chemistry).
- Phenol–chloroform_extraction wikiPageWikiLink Liquid–liquid_extraction.
- Phenol–chloroform_extraction wikiPageWikiLink Molecular_biology.
- Phenol–chloroform_extraction wikiPageWikiLink Nucleic_acid.
- Phenol–chloroform_extraction wikiPageWikiLink Phenol.
- Phenol–chloroform_extraction wikiPageWikiLink Protein.
- Phenol–chloroform_extraction wikiPageWikiLink RNA.
- Phenol–chloroform_extraction wikiPageWikiLinkText "Phenol–chloroform extraction".
- Phenol–chloroform_extraction wikiPageWikiLinkText "phenol–chloroform extraction".
- Phenol–chloroform_extraction wikiPageUsesTemplate Template:Molecular-biology-stub.
- Phenol–chloroform_extraction wikiPageUsesTemplate Template:Reflist.
- Phenol–chloroform_extraction subject Category:Biochemistry_methods.
- Phenol–chloroform_extraction subject Category:Molecular_biology.
- Phenol–chloroform_extraction hypernym Technique.
- Phenol–chloroform_extraction type TopicalConcept.
- Phenol–chloroform_extraction type Method.
- Phenol–chloroform_extraction type Process.
- Phenol–chloroform_extraction type Redirect.
- Phenol–chloroform_extraction type Technique.
- Phenol–chloroform_extraction comment "Phenol–chloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for purifying nucleic acids and eliminating proteins. In brief, aqueous samples are mixed with equal volumes of a phenol:chloroform mixture. After mixing, the mixture is centrifuged and two distinct phases are formed, because the phenol:chloroform mixture is immiscible with water. The aqueous phase is on top because it is less dense than the organic phase (phenol:chloroform).".
- Phenol–chloroform_extraction label "Phenol–chloroform extraction".
- Phenol–chloroform_extraction sameAs Q531854.
- Phenol–chloroform_extraction sameAs Phenol-Chloroform-Extraktion.
- Phenol–chloroform_extraction sameAs Extraction_au_phénol-chloroforme.
- Phenol–chloroform_extraction sameAs m.0z6qbfd.
- Phenol–chloroform_extraction sameAs Fenol-hloroformna_ekstrakcija.
- Phenol–chloroform_extraction sameAs Fenol-hloroformna_ekstrakcija.
- Phenol–chloroform_extraction sameAs Q531854.
- Phenol–chloroform_extraction wasDerivedFrom Phenol–chloroform_extraction?oldid=693943068.
- Phenol–chloroform_extraction isPrimaryTopicOf Phenol–chloroform_extraction.