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- Optical_transfection abstract "Optical transfection is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot (~1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then exposed to this highly focussed light for a small amount of time (typically tens of milliseconds to seconds), generating a transient pore on the membrane. The generation of a photopore allows exogenous plasmid DNA, RNA, organic fluorophores, or larger objects such as semiconductor quantum nanodots to enter the cell. In this technique, one cell at a time is treated, making it particularly useful for single cell analysis.To put the above simply, cells do not usually allow certain types of substances into their interior space. Lasers can be used to burn a tiny hole on the cell surface, allowing those substances to enter. This is tremendously useful to biologists who are studying disease, as a common experimental requirement is to put things (such as DNA) into cells. This technique was first demonstrated in 1984 by Tsukakoshi et al., who used a frequency tripled Nd:YAG to generate stable and transient transfection of normal rat kidney cells. Since this time, the optical transfection of a host of mammalian cell types has been demonstrated using a variety of laser sources, including the 405 nm continuous wave (cw), 488 nm cw, or pulsed sources such as the 800 nm femtosecond pulsed Ti:Sapphire or 1064 nm nanosecod pulsed Nd:YAG.".
- Optical_transfection wikiPageExternalLink optical-transfection.
- Optical_transfection wikiPageExternalLink nmeth1105-875.html.
- Optical_transfection wikiPageID "23278041".
- Optical_transfection wikiPageLength "16127".
- Optical_transfection wikiPageOutDegree "25".
- Optical_transfection wikiPageRevisionID "689348372".
- Optical_transfection wikiPageWikiLink Bacteriophage.
- Optical_transfection wikiPageWikiLink Category:Molecular_biology.
- Optical_transfection wikiPageWikiLink Cationic_liposome.
- Optical_transfection wikiPageWikiLink Cell_membrane.
- Optical_transfection wikiPageWikiLink DNA.
- Optical_transfection wikiPageWikiLink DNA_supercoil.
- Optical_transfection wikiPageWikiLink Fluorophore.
- Optical_transfection wikiPageWikiLink Growth_medium.
- Optical_transfection wikiPageWikiLink Magnet-assisted_transfection.
- Optical_transfection wikiPageWikiLink Mode-locking.
- Optical_transfection wikiPageWikiLink Nucleofection.
- Optical_transfection wikiPageWikiLink Numerical_aperture.
- Optical_transfection wikiPageWikiLink Optical_tweezers.
- Optical_transfection wikiPageWikiLink Quantum_dot.
- Optical_transfection wikiPageWikiLink RNA.
- Optical_transfection wikiPageWikiLink Small_interfering_RNA.
- Optical_transfection wikiPageWikiLink Transduction_(genetics).
- Optical_transfection wikiPageWikiLink Transfection.
- Optical_transfection wikiPageWikiLink Transformation_(genetics).
- Optical_transfection wikiPageWikiLinkText "Optical transfection".
- Optical_transfection wikiPageUsesTemplate Template:Genetic_recombination.
- Optical_transfection wikiPageUsesTemplate Template:MeshName.
- Optical_transfection wikiPageUsesTemplate Template:Reflist.
- Optical_transfection subject Category:Molecular_biology.
- Optical_transfection hypernym Process.
- Optical_transfection type Election.
- Optical_transfection type Redirect.
- Optical_transfection comment "Optical transfection is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot (~1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then exposed to this highly focussed light for a small amount of time (typically tens of milliseconds to seconds), generating a transient pore on the membrane.".
- Optical_transfection label "Optical transfection".
- Optical_transfection sameAs Q7098901.
- Optical_transfection sameAs m.0660d6w.
- Optical_transfection sameAs Q7098901.
- Optical_transfection wasDerivedFrom Optical_transfection?oldid=689348372.
- Optical_transfection isPrimaryTopicOf Optical_transfection.