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- Lysis_buffer abstract "A lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate, while detergents (such as Triton X-100 or SDS) are added to break up membrane structures. Detergent are amphipatic molecules that disrupt the hydrophobic interactions among protein domains and preserve proteins from aggregation or loss.In studies like DNA fingerprinting the lysis buffer is used for DNA isolation. Dish soap can be used in a pinch to break down the cell and nuclear membranes, allowing the DNA to be released. Other such lysis buffers include the proprietary Qiagen product Buffer P2.RIPA buffer is another commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues.".
- Lysis_buffer wikiPageID "505110".
- Lysis_buffer wikiPageLength "1582".
- Lysis_buffer wikiPageOutDegree "17".
- Lysis_buffer wikiPageRevisionID "705813260".
- Lysis_buffer wikiPageWikiLink Buffer_P2.
- Lysis_buffer wikiPageWikiLink Buffer_solution.
- Lysis_buffer wikiPageWikiLink Category:Cell_biology.
- Lysis_buffer wikiPageWikiLink Category:DNA.
- Lysis_buffer wikiPageWikiLink Category:Laboratory_techniques.
- Lysis_buffer wikiPageWikiLink DNA_profiling.
- Lysis_buffer wikiPageWikiLink Dishwashing_liquid.
- Lysis_buffer wikiPageWikiLink Ethylenediaminetetraacetic_acid.
- Lysis_buffer wikiPageWikiLink Immunoprecipitation.
- Lysis_buffer wikiPageWikiLink Lysis.
- Lysis_buffer wikiPageWikiLink Molecular_biology.
- Lysis_buffer wikiPageWikiLink Qiagen.
- Lysis_buffer wikiPageWikiLink Radioimmunoprecipitation_assay_buffer.
- Lysis_buffer wikiPageWikiLink Sodium_dodecyl_sulfate.
- Lysis_buffer wikiPageWikiLink Tris.
- Lysis_buffer wikiPageWikiLink Triton_X-100.
- Lysis_buffer wikiPageWikiLink Western_blot.
- Lysis_buffer wikiPageWikiLinkText "Lysis buffer".
- Lysis_buffer wikiPageWikiLinkText "lysis buffer".
- Lysis_buffer wikiPageUsesTemplate Template:Biology-stub.
- Lysis_buffer wikiPageUsesTemplate Template:Refimprove.
- Lysis_buffer subject Category:Cell_biology.
- Lysis_buffer subject Category:DNA.
- Lysis_buffer subject Category:Laboratory_techniques.
- Lysis_buffer hypernym Solution.
- Lysis_buffer type Software.
- Lysis_buffer type Laboratory.
- Lysis_buffer type Technique.
- Lysis_buffer comment "A lysis buffer is a buffer solution used for the purpose of lysing cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate, while detergents (such as Triton X-100 or SDS) are added to break up membrane structures.".
- Lysis_buffer label "Lysis buffer".
- Lysis_buffer sameAs Q6710242.
- Lysis_buffer sameAs m.02jgbn.
- Lysis_buffer sameAs Q6710242.
- Lysis_buffer wasDerivedFrom Lysis_buffer?oldid=705813260.
- Lysis_buffer isPrimaryTopicOf Lysis_buffer.