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- Isocaudomer abstract "Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:Mbo I N*GATC N N CTAG*NBamH I G*GATC C C CTAG*GN represents any of the four nucleotides. Independently of which nucleotide is present when cleaving with MboI, after cleavage with either enzyme, all termini have the central tetranucleotide - GATC. This allows fragments generated with one enzyme to anneal with fragments generated with the other enzyme. This can be used for elimination of restriction sites from the resulting DNA fragment. For example:Not I GC*GGCC GC CG CCGG*CGBsp120 I G*GGCC C C CCGG*GIn the above example, both enzymes produce tetranucleotides CCGG which can anneal to one another. However, resulting DNA sequence will be: GCGGCCC CGCCGGGwhere the nucleotides shown in italic originate from NotI-cut site, and those in bold from Bsp120I-cut one. Note that the resulting sequence is not recognised by either of the two enzymes.Other examples of isocaudomers include:BamHI/BclI/BglII/BstYI/DpnIINcoI/BspHI/FatI/PciINdeI/AseI/BfaI/Csp6I/MseIXbaI/AvrII/NheI/SpeI/StyIXhoI/PspXI/SalI".
- Isocaudomer wikiPageID "7172777".
- Isocaudomer wikiPageLength "1610".
- Isocaudomer wikiPageOutDegree "7".
- Isocaudomer wikiPageRevisionID "662599834".
- Isocaudomer wikiPageWikiLink Category:Enzymes.
- Isocaudomer wikiPageWikiLink Category:Restriction_enzymes.
- Isocaudomer wikiPageWikiLink GATC_(gene).
- Isocaudomer wikiPageWikiLink Nucleotide.
- Isocaudomer wikiPageWikiLink Recognition_sequence.
- Isocaudomer wikiPageWikiLink Restriction_enzyme.
- Isocaudomer wikiPageWikiLink Restriction_site.
- Isocaudomer wikiPageWikiLinkText "isocaudomer".
- Isocaudomer wikiPageUsesTemplate Template:Chem-stub.
- Isocaudomer wikiPageUsesTemplate Template:Refimprove.
- Isocaudomer wikiPageUsesTemplate Template:Reflist.
- Isocaudomer subject Category:Enzymes.
- Isocaudomer subject Category:Restriction_enzymes.
- Isocaudomer hypernym Pairs.
- Isocaudomer type FigureSkater.
- Isocaudomer comment "Isocaudomers are pairs of restriction enzymes that have slightly different recognition sequences but upon cleavage generate identical termini. For example the enzymes Mbo I and BamH I are isocaudomers:Mbo I N*GATC N N CTAG*NBamH I G*GATC C C CTAG*GN represents any of the four nucleotides. Independently of which nucleotide is present when cleaving with MboI, after cleavage with either enzyme, all termini have the central tetranucleotide - GATC.".
- Isocaudomer label "Isocaudomer".
- Isocaudomer sameAs Q17000139.
- Isocaudomer sameAs m.0h7_hy.
- Isocaudomer sameAs Q17000139.
- Isocaudomer sameAs 同尾酶.
- Isocaudomer wasDerivedFrom Isocaudomer?oldid=662599834.
- Isocaudomer isPrimaryTopicOf Isocaudomer.