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- Fast_protein_liquid_chromatography abstract "Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the \"mobile phase\") and a porous solid (the stationary phase). In FPLC the mobile phase is an aqueous solution, or \"buffer\". The buffer flow rate is controlled by a positive-displacement pump and is normally kept constant, while the composition of the buffer can be varied by drawing fluids in different proportions from two or more external reservoirs. The stationary phase is a resin composed of beads, usually of cross-linked agarose, packed into a cylindrical glass or plastic column. FPLC resins are available in a wide range of bead sizes and surface ligands depending on the application. In the most common FPLC strategy, ion exchange, a resin is chosen that the protein of interest will bind to the resin by a charge interaction while in buffer A (the running buffer) but become dissociated and return to solution in buffer B (the elution buffer). A mixture containing one or more proteins of interest is dissolved in 100% buffer A and pumped into the column. The proteins of interest bind to the resin while other components are carried out in the buffer. The total flow rate of the buffer is kept constant; however, the proportion of Buffer B (the \"elution\" buffer) is gradually increased from 0% to 100% according to a programmed change in concentration (the \"gradient\"). At some point during this process each of the bound proteins dissociates and appears in the effluent. The effluent passes through two detectors which measure salt concentration (by conductivity) and protein concentration (by absorption of ultraviolet light at a wavelength of 280nm). As each protein is eluted it appears in the effluent as a \"peak\" in protein concentration and can be collected for further use.FPLC was developed and marketed in Sweden by Pharmacia in 1982 and was originally called fast performance liquid chromatography to contrast it with HPLC or high-performance liquid chromatography. FPLC is generally applied only to proteins; however, because of the wide choice of resins and buffers it has broad applications. In contrast to HPLC the buffer pressure used is relatively low, typically less than 5 bar, but the flow rate is relatively high, typically 1-5 ml/min. FPLC can be readily scaled from analysis of milligrams of mixtures in columns with a total volume of 5ml or less to industrial production of kilograms of purified protein in columns with volumes of many liters. When used for analysis of mixtures the effluent is usually collected in fractions of 1-5 ml which can be further analyzed, e.g. by MALDI mass spectrometry. When used for protein purification there may be only two collection containers, one for the purified product and one for waste.".
- Fast_protein_liquid_chromatography thumbnail Size_Exclusion_Chromatography_Apparatus.jpg?width=300.
- Fast_protein_liquid_chromatography wikiPageExternalLink FPLCrisk.pdf.
- Fast_protein_liquid_chromatography wikiPageExternalLink 18190026.
- Fast_protein_liquid_chromatography wikiPageID "3865434".
- Fast_protein_liquid_chromatography wikiPageLength "15005".
- Fast_protein_liquid_chromatography wikiPageOutDegree "19".
- Fast_protein_liquid_chromatography wikiPageRevisionID "687817959".
- Fast_protein_liquid_chromatography wikiPageWikiLink Affinity_chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink Bio-Rad_Laboratories.
- Fast_protein_liquid_chromatography wikiPageWikiLink Category:Chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink Chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink ELISA.
- Fast_protein_liquid_chromatography wikiPageWikiLink GE_Healthcare.
- Fast_protein_liquid_chromatography wikiPageWikiLink High-performance_liquid_chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink Ion_chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink Ion_exchange.
- Fast_protein_liquid_chromatography wikiPageWikiLink ionization.
- Fast_protein_liquid_chromatography wikiPageWikiLink Nuclear_magnetic_resonance.
- Fast_protein_liquid_chromatography wikiPageWikiLink Pharmacia.
- Fast_protein_liquid_chromatography wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Fast_protein_liquid_chromatography wikiPageWikiLink Size-exclusion_chromatography.
- Fast_protein_liquid_chromatography wikiPageWikiLink X-ray_crystallography.
- Fast_protein_liquid_chromatography wikiPageWikiLinkText "FPLC".
- Fast_protein_liquid_chromatography wikiPageWikiLinkText "Fast protein liquid chromatography".
- Fast_protein_liquid_chromatography acronym "FPLC".
- Fast_protein_liquid_chromatography caption 18190026.
- Fast_protein_liquid_chromatography classification Chromatography.
- Fast_protein_liquid_chromatography manufacturers "AKTAFPLC,BioLogic Duoflow".
- Fast_protein_liquid_chromatography name "Fast protein liquid chromatography".
- Fast_protein_liquid_chromatography related Affinity_chromatography.
- Fast_protein_liquid_chromatography related High-performance_liquid_chromatography.
- Fast_protein_liquid_chromatography wikiPageUsesTemplate Template:Infobox_chemical_analysis.
- Fast_protein_liquid_chromatography wikiPageUsesTemplate Template:Reflist.
- Fast_protein_liquid_chromatography subject Category:Chromatography.
- Fast_protein_liquid_chromatography hypernym Form.
- Fast_protein_liquid_chromatography type Process.
- Fast_protein_liquid_chromatography type Technique.
- Fast_protein_liquid_chromatography comment "Fast protein liquid chromatography (FPLC), is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the \"mobile phase\") and a porous solid (the stationary phase). In FPLC the mobile phase is an aqueous solution, or \"buffer\".".
- Fast_protein_liquid_chromatography label "Fast protein liquid chromatography".
- Fast_protein_liquid_chromatography sameAs Q5437048.
- Fast_protein_liquid_chromatography sameAs m.0b3_p4.
- Fast_protein_liquid_chromatography sameAs Q5437048.
- Fast_protein_liquid_chromatography wasDerivedFrom Fast_protein_liquid_chromatography?oldid=687817959.
- Fast_protein_liquid_chromatography depiction Size_Exclusion_Chromatography_Apparatus.jpg.
- Fast_protein_liquid_chromatography isPrimaryTopicOf Fast_protein_liquid_chromatography.