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- Carboxyfluorescein_succinimidyl_ester abstract "Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells.CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within animals for many months. Subsequent studies revealed that the dye can be used to monitor lymphocyte proliferation, both in vitro and in vivo, due to the progressive halving of CFSE fluorescence within daughter cells following each cell division. The only limitation is that CFSE at high concentrations can be toxic for cells. However, when CFSE labelling is performed optimally, approximately 7-8 cell divisions can be identified before the CFSE fluorescence is too low to be distinguished above the autofluorescence background. Thus CFSE represents an extremely valuable fluorescent dye for immunological studies, allowing lymphocyte proliferation, migration and positioning to be simultaneously monitored. By the use of fluorescent antibodies against different lymphocyte cell surface markers it is also possible to follow the proliferation behaviour of different lymphocyte subsets. In addition, unlike other methods, CFSE-labeled viable cells can be recovered for further analysis.Since the initial description of CFSE it has been used in thousands of immunological studies, an example of an early proliferation study in animals being described by Kurts et al. However, perhaps the most important CFSE investigations have been those demonstrating that many of the effector functions of lymphocytes, such as cytokine production by T lymphocytes, and antibody class switching by B cells, are division dependent. Sophisticated mathematical models have also been developed to analyse CFSE data and probe various aspects of immune responses. Furthermore, the use of CFSE has extended beyond the immune system, with the dye being used to monitor the proliferation of many other cell types such as smooth muscle cells, fibroblasts, hematopoietic stem cells and even bacteria. Another novel application of CFSE is its use for the in vitro and in vivo determination of cytotoxic lymphocytes.Detailed protocols are now available that can be used to label lymphocytes (and other cell types) with a high degree of reliability and precision. One of the most important parameters, however, is to ensure that the cell population being studied has not been too heavily labelled with CFSE as such cells, although remaining viable, proliferate sub-optimally.".
- Carboxyfluorescein_succinimidyl_ester thumbnail Carboxyfluorescein_succinimidyl_ester.png?width=300.
- Carboxyfluorescein_succinimidyl_ester wikiPageID "9384017".
- Carboxyfluorescein_succinimidyl_ester wikiPageLength "13483".
- Carboxyfluorescein_succinimidyl_ester wikiPageOutDegree "21".
- Carboxyfluorescein_succinimidyl_ester wikiPageRevisionID "660029979".
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink B_cell.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Carboxyfluorescein_diacetate_succinimidyl_ester.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Category:Flow_cytometry.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Category:Fluorescent_dyes.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Category:Fluorone_dyes.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Category:Succinimides.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Cell_(biology).
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Cell_growth.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Cytokine.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Cytoplasm.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Cytotoxic_T_cell.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Fibroblast.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Fluorescence.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Hematopoietic_stem_cell.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Immunoglobulin_class_switching.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Lymphocyte.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Lysine.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink N-Hydroxysuccinimide.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Smooth_muscle_tissue.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink Staining.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLink T_cell.
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLinkText "CFSE".
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLinkText "Carboxyfluorescein succinimidyl ester".
- Carboxyfluorescein_succinimidyl_ester wikiPageWikiLinkText "carboxyfluorescein succinimidyl ester".
- Carboxyfluorescein_succinimidyl_ester imagefile "Carboxyfluorescein succinimidyl ester.png".
- Carboxyfluorescein_succinimidyl_ester name "6".
- Carboxyfluorescein_succinimidyl_ester othernames "CFSE; Carboxyfluorescein N-succinimidyl ester".
- Carboxyfluorescein_succinimidyl_ester verifiedfields "changed".
- Carboxyfluorescein_succinimidyl_ester verifiedrevid "439546511".
- Carboxyfluorescein_succinimidyl_ester watchedfields "changed".
- Carboxyfluorescein_succinimidyl_ester wikiPageUsesTemplate Template:About.
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- Carboxyfluorescein_succinimidyl_ester subject Category:Flow_cytometry.
- Carboxyfluorescein_succinimidyl_ester subject Category:Fluorescent_dyes.
- Carboxyfluorescein_succinimidyl_ester subject Category:Fluorone_dyes.
- Carboxyfluorescein_succinimidyl_ester subject Category:Succinimides.
- Carboxyfluorescein_succinimidyl_ester hypernym Cell.
- Carboxyfluorescein_succinimidyl_ester type AnatomicalStructure.
- Carboxyfluorescein_succinimidyl_ester type ChemicalCompound.
- Carboxyfluorescein_succinimidyl_ester type ChemicalSubstance.
- Carboxyfluorescein_succinimidyl_ester type Chemical.
- Carboxyfluorescein_succinimidyl_ester type Imide.
- Carboxyfluorescein_succinimidyl_ester type Technique.
- Carboxyfluorescein_succinimidyl_ester type ChemicalObject.
- Carboxyfluorescein_succinimidyl_ester type Thing.
- Carboxyfluorescein_succinimidyl_ester type Q11173.
- Carboxyfluorescein_succinimidyl_ester comment "Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods.".
- Carboxyfluorescein_succinimidyl_ester label "Carboxyfluorescein succinimidyl ester".
- Carboxyfluorescein_succinimidyl_ester sameAs Q4035612.
- Carboxyfluorescein_succinimidyl_ester sameAs Estere_succinimmidico_della_carbossifluoresceina.
- Carboxyfluorescein_succinimidyl_ester sameAs m.0286q6g.
- Carboxyfluorescein_succinimidyl_ester sameAs CFSE.
- Carboxyfluorescein_succinimidyl_ester sameAs Karboksifluorescein_sukcinimidil_estar.
- Carboxyfluorescein_succinimidyl_ester sameAs Karboksifluorescein_sukcinimidil_estar.
- Carboxyfluorescein_succinimidyl_ester sameAs Q4035612.
- Carboxyfluorescein_succinimidyl_ester wasDerivedFrom Carboxyfluorescein_succinimidyl_ester?oldid=660029979.
- Carboxyfluorescein_succinimidyl_ester depiction Carboxyfluorescein_succinimidyl_ester.png.
- Carboxyfluorescein_succinimidyl_ester isPrimaryTopicOf Carboxyfluorescein_succinimidyl_ester.
- Carboxyfluorescein_succinimidyl_ester name "6-Carboxyfluorescein succinimidyl ester".