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- Terminal_amine_isotopic_labeling_of_substrates abstract "Terminal amine isotopic labeling of substrates (TAILS) is a method in quantitative proteomics that identifies the protein content of samples based on N-terminal fragments of each protein (N-terminal peptides) and detects differences in protein abundance among samples.Like other methods based on N-terminal peptides, this assay uses trypsin to break proteins into fragments and separates the N-terminal peptides (the fragments containing the N-termini of the original proteins) from the other fragments (internal tryptic peptides). TAILS isolates the N-terminal peptides by identifying and removing the internal tryptic peptides. This negative selection allows the TAILS method to detect all N-termini in the given samples. Alternative methods that rely on the free amino group of the N-terminus to identify the N-terminal peptides cannot detect some N-termini because they are "naturally blocked" (i.e. the natural protein does not have a free amino group).The TAILS method has a number of applications including the identification of new substrates and proteases (including those that have an unknown and broad specificity) and as a way to define the termini of proteins that enables protein annotation. TAILS can also be used to link proteases with a variety of defined biological pathways in diseases such as cancer, in order to gain a clearer understanding of the substrates and proteases involved in the disease state.".
- Terminal_amine_isotopic_labeling_of_substrates thumbnail TAILS_overview_picture.JPG?width=300.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageID "34930324".
- Terminal_amine_isotopic_labeling_of_substrates wikiPageLength "15522".
- Terminal_amine_isotopic_labeling_of_substrates wikiPageOutDegree "29".
- Terminal_amine_isotopic_labeling_of_substrates wikiPageRevisionID "632377284".
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Bioinformatics.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Cancer.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Category:Mass_spectrometry.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Category:Proteomics.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Elution.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Ex_vivo.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Formaldehyde.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink ITRAQ.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink In_vivo.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Isobaric_tag_for_relative_and_absolute_quantification.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Isobaric_tag_for_relative_and_absolute_quantitation.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Isotope.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Mass_spectrometry.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Metabolic.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Metabolism.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Multiplex_(assay).
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink N-terminus.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Peptide.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Peptides.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Protease.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Proteases.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Protein.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Proteolysis.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Quantitative_proteomics.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink SILAC.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Stable_isotope_labeling_by_amino_acids_in_cell_culture.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Substrate_(biochemistry).
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Substrate_(chemistry).
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Tandem_mass_spectrometry.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Trypsin.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink Ultrafiltration.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLink University_of_British_Columbia.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageWikiLinkText "Terminal amine isotopic labeling of substrates".
- Terminal_amine_isotopic_labeling_of_substrates alt "Terminal amine isotopic labeling of substrates overview".
- Terminal_amine_isotopic_labeling_of_substrates annotColor "red".
- Terminal_amine_isotopic_labeling_of_substrates annotFontWeight "bold".
- Terminal_amine_isotopic_labeling_of_substrates caption "TAILS Procedure Overview. Numbers in red identify steps in the text.".
- Terminal_amine_isotopic_labeling_of_substrates date "August 2014".
- Terminal_amine_isotopic_labeling_of_substrates hasPhotoCollection Terminal_amine_isotopic_labeling_of_substrates.
- Terminal_amine_isotopic_labeling_of_substrates imageWidth "498".
- Terminal_amine_isotopic_labeling_of_substrates reason "why does lack of independence mean they cannot be averaged and why is this bad?".
- Terminal_amine_isotopic_labeling_of_substrates wikiPageUsesTemplate Template:Annotated_image.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageUsesTemplate Template:Clarify.
- Terminal_amine_isotopic_labeling_of_substrates wikiPageUsesTemplate Template:Reflist.
- Terminal_amine_isotopic_labeling_of_substrates subject Category:Mass_spectrometry.
- Terminal_amine_isotopic_labeling_of_substrates subject Category:Proteomics.
- Terminal_amine_isotopic_labeling_of_substrates hypernym Method.
- Terminal_amine_isotopic_labeling_of_substrates type Article.
- Terminal_amine_isotopic_labeling_of_substrates type Software.
- Terminal_amine_isotopic_labeling_of_substrates type Article.
- Terminal_amine_isotopic_labeling_of_substrates type Omic.
- Terminal_amine_isotopic_labeling_of_substrates type Proteomic.
- Terminal_amine_isotopic_labeling_of_substrates comment "Terminal amine isotopic labeling of substrates (TAILS) is a method in quantitative proteomics that identifies the protein content of samples based on N-terminal fragments of each protein (N-terminal peptides) and detects differences in protein abundance among samples.Like other methods based on N-terminal peptides, this assay uses trypsin to break proteins into fragments and separates the N-terminal peptides (the fragments containing the N-termini of the original proteins) from the other fragments (internal tryptic peptides). ".
- Terminal_amine_isotopic_labeling_of_substrates label "Terminal amine isotopic labeling of substrates".
- Terminal_amine_isotopic_labeling_of_substrates sameAs m.0j440vn.
- Terminal_amine_isotopic_labeling_of_substrates sameAs Q7702707.
- Terminal_amine_isotopic_labeling_of_substrates sameAs Q7702707.
- Terminal_amine_isotopic_labeling_of_substrates wasDerivedFrom Terminal_amine_isotopic_labeling_of_substrates?oldid=632377284.
- Terminal_amine_isotopic_labeling_of_substrates depiction TAILS_overview_picture.JPG.
- Terminal_amine_isotopic_labeling_of_substrates isPrimaryTopicOf Terminal_amine_isotopic_labeling_of_substrates.