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- Restriction_fragment abstract "A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site. Most restriction sites are palindromic, (the sequence of nucleotides is the same on both strands when read in the 5' to 3' direction), and are four to eight nucleotides long. Many cuts are made by one restriction enzyme because of the chance repetition of these sequences in a long DNA molecule, yielding a set of restriction fragments. A particular DNA molecule will always yield the same set of restriction fragments when exposed to the same restriction enzyme. Restriction fragments can be analyzed using techniques such as gel electrophoresis or used in recombinant DNA technology.In recombinant DNA technology specific restriction endonucleases are used that will isolate a particular gene and cleave the sugar phosphate backbones at different points (retaining symmetry), so that the double stranded restriction fragments have single stranded ends. These short extensions, called sticky ends can form hydrogen bonded base pairs with complementary sticky ends on any other DNA cut with the same enzyme (such as a bacterial plasmid).In agarose gel electrophoresis, the restriction fragments yield a band pattern characteristic of the original DNA molecule and restriction enzyme used, for example the relatively small DNA molecules of viruses and plasmids can be identified simply by their restriction fragment patterns. If the nucleotide differences of two different alleles occur within the restriction site of a particular restriction enzyme, digestion of segments of DNA from individuals with different alleles for that particular gene with that enzyme would produce different fragments and that will each yield different band patterns in gel electrophoresis.".
- Restriction_fragment thumbnail Restriction_enzyme.jpg?width=300.
- Restriction_fragment wikiPageID "2941387".
- Restriction_fragment wikiPageLength "2511".
- Restriction_fragment wikiPageOutDegree "13".
- Restriction_fragment wikiPageRevisionID "618209936".
- Restriction_fragment wikiPageWikiLink Agarose_gel_electrophoresis.
- Restriction_fragment wikiPageWikiLink Allele.
- Restriction_fragment wikiPageWikiLink Category:Molecular_biology.
- Restriction_fragment wikiPageWikiLink Category:Restriction_enzymes.
- Restriction_fragment wikiPageWikiLink DNA.
- Restriction_fragment wikiPageWikiLink Gel_electrophoresis.
- Restriction_fragment wikiPageWikiLink Hydrogen_bond.
- Restriction_fragment wikiPageWikiLink Nucleotide.
- Restriction_fragment wikiPageWikiLink Palindromic_sequence.
- Restriction_fragment wikiPageWikiLink Recombinant_DNA.
- Restriction_fragment wikiPageWikiLink Restriction_enzyme.
- Restriction_fragment wikiPageWikiLink Sticky_and_blunt_ends.
- Restriction_fragment wikiPageWikiLink File:Restriction_enzyme.jpg.
- Restriction_fragment wikiPageWikiLinkText "Restriction fragment".
- Restriction_fragment wikiPageWikiLinkText "restriction fragment".
- Restriction_fragment hasPhotoCollection Restriction_fragment.
- Restriction_fragment wikiPageUsesTemplate Template:Cite_book.
- Restriction_fragment wikiPageUsesTemplate Template:Genetics-stub.
- Restriction_fragment subject Category:Molecular_biology.
- Restriction_fragment subject Category:Restriction_enzymes.
- Restriction_fragment hypernym Fragment.
- Restriction_fragment type Drug.
- Restriction_fragment comment "A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.".
- Restriction_fragment label "Restriction fragment".
- Restriction_fragment sameAs m.08f379.
- Restriction_fragment sameAs Q7316314.
- Restriction_fragment sameAs Q7316314.
- Restriction_fragment wasDerivedFrom Restriction_fragment?oldid=618209936.
- Restriction_fragment depiction Restriction_enzyme.jpg.
- Restriction_fragment isPrimaryTopicOf Restriction_fragment.
