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- Pyrosequencing abstract "Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides. The technique was developed by Mostafa Ronaghi and Pål Nyrén at the Royal Institute of Technology in Stockholm in 1996.The desired DNA sequence is able to be determined by light emitted upon incorporation of the next complementary nucleotide by the fact that only one out of four of the possible A/T/C/G nucleotides are added and available at a time so that only one letter can be incorporated on the single stranded template (which is the sequence to be determined). The intensity of the light determines if there are more than one of these "letters" in a row. The previous nucleotide letter (one out of four possible dNTP) is degraded before the next nucleotide letter is added for synthesis: allowing for the possible revealing of the next nucleotide(s) via the resulting intensity of light (if the nucleotide added was the next complementary letter in the sequence). This process is repeated with each of the four letters until the DNA sequence of the single stranded template is determined.".
- Pyrosequencing thumbnail Pyrogram1.jpg?width=300.
- Pyrosequencing wikiPageID "1154853".
- Pyrosequencing wikiPageLength "7650".
- Pyrosequencing wikiPageOutDegree "48".
- Pyrosequencing wikiPageRevisionID "672751996".
- Pyrosequencing wikiPageWikiLink 3-Phosphoadenosine-5-phosphosulfate.
- Pyrosequencing wikiPageWikiLink 454_Life_Sciences.
- Pyrosequencing wikiPageWikiLink ATP_sulfurylase.
- Pyrosequencing wikiPageWikiLink Adenosine_5´_phosphosulfate.
- Pyrosequencing wikiPageWikiLink Adenosine_triphosphate.
- Pyrosequencing wikiPageWikiLink Apyrase.
- Pyrosequencing wikiPageWikiLink Category:Biotechnology.
- Pyrosequencing wikiPageWikiLink Category:DNA_sequencing.
- Pyrosequencing wikiPageWikiLink Category:Life_sciences_industry.
- Pyrosequencing wikiPageWikiLink Category:Molecular_biology.
- Pyrosequencing wikiPageWikiLink Chemiluminescence.
- Pyrosequencing wikiPageWikiLink DNA.
- Pyrosequencing wikiPageWikiLink DNA_polymerase.
- Pyrosequencing wikiPageWikiLink DNA_sequencing.
- Pyrosequencing wikiPageWikiLink DNTP.
- Pyrosequencing wikiPageWikiLink Deoxynucleoside_triphosphate.
- Pyrosequencing wikiPageWikiLink Dideoxynucleotide.
- Pyrosequencing wikiPageWikiLink Dideoxynucleotides.
- Pyrosequencing wikiPageWikiLink Enzyme.
- Pyrosequencing wikiPageWikiLink Exonuclease.
- Pyrosequencing wikiPageWikiLink Genome_assembly.
- Pyrosequencing wikiPageWikiLink Genome_project.
- Pyrosequencing wikiPageWikiLink HealthCap.
- Pyrosequencing wikiPageWikiLink Luciferase.
- Pyrosequencing wikiPageWikiLink Luciferin.
- Pyrosequencing wikiPageWikiLink Metagenomics.
- Pyrosequencing wikiPageWikiLink Mostafa_Ronaghi.
- Pyrosequencing wikiPageWikiLink Nucleoside_triphosphate.
- Pyrosequencing wikiPageWikiLink Nucleotide.
- Pyrosequencing wikiPageWikiLink Nucleotides.
- Pyrosequencing wikiPageWikiLink Polymerase_chain_reaction.
- Pyrosequencing wikiPageWikiLink Primer_(molecular_biology).
- Pyrosequencing wikiPageWikiLink Pyrophosphate.
- Pyrosequencing wikiPageWikiLink Pål_Nyrén.
- Pyrosequencing wikiPageWikiLink Repeated_sequence_(DNA).
- Pyrosequencing wikiPageWikiLink Repetitive_DNA.
- Pyrosequencing wikiPageWikiLink Roche_Diagnostics.
- Pyrosequencing wikiPageWikiLink Royal_Institute_of_Technology.
- Pyrosequencing wikiPageWikiLink Sanger_sequencing.
- Pyrosequencing wikiPageWikiLink SsDNA.
- Pyrosequencing wikiPageWikiLink Stockholm_Stock_Exchange.
- Pyrosequencing wikiPageWikiLink Streptavidin.
- Pyrosequencing wikiPageWikiLink Sulfate_adenylyltransferase.
- Pyrosequencing wikiPageWikiLink Uppsala.
- Pyrosequencing wikiPageWikiLink Uppsala,_Sweden.
- Pyrosequencing wikiPageWikiLink Venture_capital.
- Pyrosequencing wikiPageWikiLink File:How_Pyrosequencing_Works.svg.
- Pyrosequencing wikiPageWikiLink File:Pyrogram1.jpg.
- Pyrosequencing wikiPageWikiLinkText "454 pyrosequencing".
- Pyrosequencing wikiPageWikiLinkText "Pyrosequencing".
- Pyrosequencing wikiPageWikiLinkText "Pyrosequencing#Licensing".
- Pyrosequencing wikiPageWikiLinkText "Roche/454 Pyro-sequencing".
- Pyrosequencing wikiPageWikiLinkText "pyrosequencing".
- Pyrosequencing wikiPageWikiLinkText "sequencing-by-synthesis".
- Pyrosequencing hasPhotoCollection Pyrosequencing.
- Pyrosequencing wikiPageUsesTemplate Template:As_of.
- Pyrosequencing wikiPageUsesTemplate Template:Cite_journal.
- Pyrosequencing wikiPageUsesTemplate Template:More_footnotes.
- Pyrosequencing wikiPageUsesTemplate Template:Reflist.
- Pyrosequencing subject Category:Biotechnology.
- Pyrosequencing subject Category:DNA_sequencing.
- Pyrosequencing subject Category:Life_sciences_industry.
- Pyrosequencing subject Category:Molecular_biology.
- Pyrosequencing hypernym Method.
- Pyrosequencing type Article.
- Pyrosequencing type Software.
- Pyrosequencing type Article.
- Pyrosequencing comment "Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides.".
- Pyrosequencing label "Pyrosequencing".
- Pyrosequencing sameAs سلسلة_البايرو.
- Pyrosequencing sameAs Piroseqüenciació.
- Pyrosequencing sameAs Pyrosekvenování.
- Pyrosequencing sameAs Pirosecuenciación.
- Pyrosequencing sameAs Pyroséquençage.
- Pyrosequencing sameAs Pyrosequencing.
- Pyrosequencing sameAs Pyrosekvensering.
- Pyrosequencing sameAs Pirosequenciamento.
- Pyrosequencing sameAs m.04bw66.
- Pyrosequencing sameAs Пиросеквенирование.
- Pyrosequencing sameAs Pyrosekvensering.
- Pyrosequencing sameAs Піросеквенування.
- Pyrosequencing sameAs Q2342248.
- Pyrosequencing sameAs Q2342248.
- Pyrosequencing sameAs 焦磷酸测序.
- Pyrosequencing wasDerivedFrom Pyrosequencing?oldid=672751996.
- Pyrosequencing depiction Pyrogram1.jpg.
- Pyrosequencing isPrimaryTopicOf Pyrosequencing.