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- Primer_walking abstract "Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome. The term "primer walking" is used where the main aim is to sequence the genome. The term "chromosome walking" is used instead when we know the sequence but don't have a clone of a gene. For example the gene for a disease may be located near a specific marker such as an RFLP on the sequence.The fragment is first sequenced as if it were a shorter fragment — sequencing will be performed from each end using either universal primers or primers designated by the customer. This should identify the first 1000 (approx.) bases. In order to completely sequence the region of interest, design and synthesis of new primers — complementary to the final 20 bases of the known sequence — is necessary to obtain contiguous sequence information.The basic technique is as follows: A primer that matches the beginning of the DNA to sequence is used to synthesize a short DNA strand adjacent to the unknown sequence, starting with the primer (see PCR). The new short DNA strand is sequenced using the chain termination method. The end of the sequenced strand is used as a primer for the next part of the long DNA sequence.That way, the short part of the long DNA that is sequenced keeps "walking" along the sequence. The method can be used to sequence entire chromosomes (thus, chromosome walking). A different method with the same purpose which becomes more popular for large-scale sequencing (e.g., the Human Genome Project) is shotgun sequencing.".
- Primer_walking wikiPageExternalLink 390520243e7640e689a3e40286806059.
- Primer_walking wikiPageID "1484989".
- Primer_walking wikiPageLength "3000".
- Primer_walking wikiPageOutDegree "18".
- Primer_walking wikiPageRevisionID "664374867".
- Primer_walking wikiPageWikiLink Base_pair.
- Primer_walking wikiPageWikiLink Category:DNA.
- Primer_walking wikiPageWikiLink Category:Molecular_biology.
- Primer_walking wikiPageWikiLink Chain_termination_method.
- Primer_walking wikiPageWikiLink Chemical_synthesis.
- Primer_walking wikiPageWikiLink Chromosome_jumping.
- Primer_walking wikiPageWikiLink Chromosome_landing.
- Primer_walking wikiPageWikiLink DNA.
- Primer_walking wikiPageWikiLink Human_Genome_Project.
- Primer_walking wikiPageWikiLink Kilobase.
- Primer_walking wikiPageWikiLink PCR.
- Primer_walking wikiPageWikiLink Plasmid.
- Primer_walking wikiPageWikiLink Polymerase_chain_reaction.
- Primer_walking wikiPageWikiLink Primer_(molecular_biology).
- Primer_walking wikiPageWikiLink RFLP.
- Primer_walking wikiPageWikiLink Restriction_fragment_length_polymorphism.
- Primer_walking wikiPageWikiLink Sanger_sequencing.
- Primer_walking wikiPageWikiLink Sequencing.
- Primer_walking wikiPageWikiLink Shotgun_sequencing.
- Primer_walking wikiPageWikiLinkText "Chromosome Walking".
- Primer_walking wikiPageWikiLinkText "Primer walking".
- Primer_walking wikiPageWikiLinkText "chromosome walking".
- Primer_walking wikiPageWikiLinkText "primer walk".
- Primer_walking wikiPageWikiLinkText "primer walking".
- Primer_walking hasPhotoCollection Primer_walking.
- Primer_walking wikiPageUsesTemplate Template:Cite_journal.
- Primer_walking wikiPageUsesTemplate Template:Molecular-biology-stub.
- Primer_walking wikiPageUsesTemplate Template:Refimprove.
- Primer_walking wikiPageUsesTemplate Template:Reflist.
- Primer_walking subject Category:DNA.
- Primer_walking subject Category:Molecular_biology.
- Primer_walking hypernym Method.
- Primer_walking type Article.
- Primer_walking type Software.
- Primer_walking type Article.
- Primer_walking comment "Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome. The term "primer walking" is used where the main aim is to sequence the genome.".
- Primer_walking label "Primer walking".
- Primer_walking sameAs Walking.
- Primer_walking sameAs Arpentage_chromosomique.
- Primer_walking sameAs Spacer_po_chromosomie.
- Primer_walking sameAs m.0554gc.
- Primer_walking sameAs Q3817120.
- Primer_walking sameAs Q3817120.
- Primer_walking wasDerivedFrom Primer_walking?oldid=664374867.
- Primer_walking isPrimaryTopicOf Primer_walking.