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- Myc-tag abstract "A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.The peptide sequence of the myc-tag is (in 1- and 3-letter codes, respectively): N-EQKLISEEDL-C, N-Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.A monoclonal antibody against the myc epitope, named 9E10, is available from the non-commercial Developmental Studies Hybridoma Bank.".
- Myc-tag wikiPageID "21093613".
- Myc-tag wikiPageLength "1722".
- Myc-tag wikiPageOutDegree "18".
- Myc-tag wikiPageRevisionID "605632092".
- Myc-tag wikiPageWikiLink Affinity_chromatography.
- Myc-tag wikiPageWikiLink Amino-terminus.
- Myc-tag wikiPageWikiLink Antibody.
- Myc-tag wikiPageWikiLink Atomic_mass_unit.
- Myc-tag wikiPageWikiLink C-myc.
- Myc-tag wikiPageWikiLink C-terminus.
- Myc-tag wikiPageWikiLink Carboxy_terminus.
- Myc-tag wikiPageWikiLink Category:Biochemistry.
- Myc-tag wikiPageWikiLink Dalton_(unit).
- Myc-tag wikiPageWikiLink Developmental_Studies_Hybridoma_Bank.
- Myc-tag wikiPageWikiLink Epitope.
- Myc-tag wikiPageWikiLink FLAG-tag.
- Myc-tag wikiPageWikiLink Flag_tag.
- Myc-tag wikiPageWikiLink Myc.
- Myc-tag wikiPageWikiLink N-terminus.
- Myc-tag wikiPageWikiLink Polyhistidine-tag.
- Myc-tag wikiPageWikiLink Protein_tag.
- Myc-tag wikiPageWikiLink Recombinant_DNA.
- Myc-tag wikiPageWikiLink Secretory_pathway.
- Myc-tag wikiPageWikiLink Western_blot.
- Myc-tag wikiPageWikiLink Western_blotting.
- Myc-tag wikiPageWikiLinkText "Myc-tag".
- Myc-tag wikiPageWikiLinkText "myc-tag".
- Myc-tag hasPhotoCollection Myc-tag.
- Myc-tag subject Category:Biochemistry.
- Myc-tag hypernym Tag.
- Myc-tag type Person.
- Myc-tag comment "A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A myc tag can be used in many different assays that require recognition by an antibody.".
- Myc-tag label "Myc-tag".
- Myc-tag sameAs m.05c232h.
- Myc-tag sameAs Q6946829.
- Myc-tag sameAs Q6946829.
- Myc-tag wasDerivedFrom Myc-tag?oldid=605632092.
- Myc-tag isPrimaryTopicOf Myc-tag.