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- LB_buffer abstract "LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate (lithium hydroxide monohydrate and boric acid). LB(R) is a registered (USPTO) trademark of Faster Better Media LLC, which owns US patent 7,163,610 covering low-conductance lithium borate polynucleotide electrophoresis. Lithium Borate buffer has a lower conductivity, produces crisper resolution, and can be run at higher speeds than can gels made from TBE or TAE (5-50 V/cm as compared to 5-10 V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TBE/TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected with lithium boric acid gels. SB buffer containing sodium borate is similar to lithium borate and has nearly all of its advantages at a somewhat lower cost, but the lithium buffer permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions.".
- LB_buffer wikiPageExternalLink borate.html.
- LB_buffer wikiPageID "14841597".
- LB_buffer wikiPageLength "2452".
- LB_buffer wikiPageOutDegree "17".
- LB_buffer wikiPageRevisionID "499296526".
- LB_buffer wikiPageWikiLink Borax.
- LB_buffer wikiPageWikiLink Boric_acid.
- LB_buffer wikiPageWikiLink Buffer_solution.
- LB_buffer wikiPageWikiLink Category:Buffers.
- LB_buffer wikiPageWikiLink Conductivity_(electrolytic).
- LB_buffer wikiPageWikiLink DNA.
- LB_buffer wikiPageWikiLink Electrophoresis.
- LB_buffer wikiPageWikiLink Lithium_borate.
- LB_buffer wikiPageWikiLink Lithium_hydroxide.
- LB_buffer wikiPageWikiLink Nucleic_acid.
- LB_buffer wikiPageWikiLink Nucleic_acids.
- LB_buffer wikiPageWikiLink RNA.
- LB_buffer wikiPageWikiLink SB_buffer.
- LB_buffer wikiPageWikiLink Sodium_borate.
- LB_buffer wikiPageWikiLink Southern_blot.
- LB_buffer wikiPageWikiLink TAE_buffer.
- LB_buffer wikiPageWikiLink TBE_buffer.
- LB_buffer wikiPageWikiLinkText "LB buffer".
- LB_buffer wikiPageWikiLinkText "Lithium boric acid".
- LB_buffer wikiPageWikiLinkText "lithium borate".
- LB_buffer hasPhotoCollection LB_buffer.
- LB_buffer wikiPageUsesTemplate Template:Biochem-stub.
- LB_buffer subject Category:Buffers.
- LB_buffer hypernym Solution.
- LB_buffer type Software.
- LB_buffer comment "LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Lithium borate (lithium hydroxide monohydrate and boric acid). LB(R) is a registered (USPTO) trademark of Faster Better Media LLC, which owns US patent 7,163,610 covering low-conductance lithium borate polynucleotide electrophoresis.".
- LB_buffer label "LB buffer".
- LB_buffer sameAs Lithiumborat-Puffer.
- LB_buffer sameAs m.03gzt79.
- LB_buffer sameAs Q6457326.
- LB_buffer sameAs Q6457326.
- LB_buffer wasDerivedFrom LB_buffer?oldid=499296526.
- LB_buffer isPrimaryTopicOf LB_buffer.