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- Isothermal_microcalorimetry abstract "Isothermal microcalorimetry (IMC) is a laboratory method for real-time monitoring and dynamic analysis of chemical, physical and biological processes. Over a period of hours or days, IMC determines the onset, rate, extent and energetics of such processes for specimens in small ampoules (e.g. 3–20 ml) at a constant set temperature (ca. 15°C–150°C).IMC accomplishes this dynamic analysis by measuring and recording vs. elapsed time the net rate of heat flow (μJ/sec = μW) to or from the specimen ampoule, and the cumulative amount of heat (J) consumed or produced.IMC is a powerful and versatile analytical tool for four closely related reasons: All chemical and physical processes are either exothermic or endothermic—produce or consume heat. The rate of heat flow is proportional to the rate of the process taking place. IMC is sensitive enough to detect and follow either slow processes (reactions proceeding at a few % per year) in a few grams of material, or processes which generate minuscule amounts of heat (e.g. metabolism of a few thousand living cells). IMC instruments generally have a huge dynamic range—heat flows as low as ca. 1 μW and as high as ca. 50,000 μW can be measured by the same instrument.The IMC method of studying rates of processes is thus broadly applicable, provides real-time continuous data, and is sensitive. The measurement is simple to make, takes place unattended and is non-interfering (e.g. no fluorescent or radioactive markers are needed).However, there are two main caveats that must be heeded in use of IMC: Missed data: If externally prepared specimen ampoules are used, it takes ca. 40 minutes to slowly introduce an ampoule into the instrument without significant disturbance of the set temperature in the measurement module. Thus any processes taking place during this time are not monitored. Extraneous data: IMC records the aggregate net heat flow produced or consumed by all processes taking place within an ampoule. Therefore, in order to be sure what process or processes are producing the measured heat flow, great care must be taken in both experimental design and in the initial use of related chemical, physical and biologic assays.In general, possible applications of IMC are only limited by the imagination of the person who chooses to employ it as an analytical tool and the physical constraints of the method. Besides the two general limitations (main caveats) described above, these constraints include specimen and ampoule size, and the temperatures at which measurements can be made. IMC is generally best suited to evaluating processes which take place over hours or days. IMC has been used in an extremely wide range of applications, and many examples are discussed in this article, supported by references to published literature. Applications discussed range from measurement of slow oxidative degradation of polymers and instability of hazardous industrial chemicals to detection of bacteria in urine and evaluation of the effects of drugs on parasitic worms. The present emphasis in this article is applications of the latter type—biology and medicine.".
- Isothermal_microcalorimetry thumbnail An_artists_rendition_of_an_isothermal_microcalorimetry_(IMC)_instrument_and_one_of_its_measurement_units.jpgwidth=300.
- Isothermal_microcalorimetry wikiPageExternalLink www.calmetrix.com.
- Isothermal_microcalorimetry wikiPageExternalLink instruments.html.
- Isothermal_microcalorimetry wikiPageExternalLink Microcalorimetry.htm.
- Isothermal_microcalorimetry wikiPageExternalLink www.symcel.se.
- Isothermal_microcalorimetry wikiPageExternalLink www.tainstruments.com.
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- Isothermal_microcalorimetry wikiPageRevisionID "674049784".
- Isothermal_microcalorimetry wikiPageWikiLink Adipocyte.
- Isothermal_microcalorimetry wikiPageWikiLink Adipocytes.
- Isothermal_microcalorimetry wikiPageWikiLink Ampoule.
- Isothermal_microcalorimetry wikiPageWikiLink Bacterial_growth.
- Isothermal_microcalorimetry wikiPageWikiLink Bactericide.
- Isothermal_microcalorimetry wikiPageWikiLink Bacteriostatic_agent.
- Isothermal_microcalorimetry wikiPageWikiLink Calcium_hydroxyapatite.
- Isothermal_microcalorimetry wikiPageWikiLink Calorimeter.
- Isothermal_microcalorimetry wikiPageWikiLink Calorimetry.
- Isothermal_microcalorimetry wikiPageWikiLink Category:Biological_processes.
- Isothermal_microcalorimetry wikiPageWikiLink Category:Calorimetry.
- Isothermal_microcalorimetry wikiPageWikiLink Category:Chemical_processes.
- Isothermal_microcalorimetry wikiPageWikiLink Category:Heat_transfer.
- Isothermal_microcalorimetry wikiPageWikiLink Chemical_thermodynamics.
- Isothermal_microcalorimetry wikiPageWikiLink Chondrocyte.
- Isothermal_microcalorimetry wikiPageWikiLink Chondrocytes.
- Isothermal_microcalorimetry wikiPageWikiLink Coupon_(materials_science).
- Isothermal_microcalorimetry wikiPageWikiLink Differential_scanning_calorimetry.
- Isothermal_microcalorimetry wikiPageWikiLink Erythrocytes.
- Isothermal_microcalorimetry wikiPageWikiLink File:Isothermal_microcalorimetry_(IMC)_measurement_of_heat_flow_from_fibroblasts_in_suspension,_due_to_injection_of_sodium_dodecyl_sulfate.jpg.
- Isothermal_microcalorimetry wikiPageWikiLink File:Isothermal_microcalorimetry_(IMC)_measurements_of_heat_flow_vs._time_for_80%25_cumene_hydroperoxide.jpg.
- Isothermal_microcalorimetry wikiPageWikiLink Food_science.
- Isothermal_microcalorimetry wikiPageWikiLink Germination.
- Isothermal_microcalorimetry wikiPageWikiLink Granulocyte.
- Isothermal_microcalorimetry wikiPageWikiLink Granulocytes.
- Isothermal_microcalorimetry wikiPageWikiLink Hydroxylapatite.
- Isothermal_microcalorimetry wikiPageWikiLink IR_spectroscopy.
- Isothermal_microcalorimetry wikiPageWikiLink Infrared_spectroscopy.
- Isothermal_microcalorimetry wikiPageWikiLink Isothermal_titration_calorimetry.
- Isothermal_microcalorimetry wikiPageWikiLink Lymphocyte.
- Isothermal_microcalorimetry wikiPageWikiLink Lymphocytes.
- Isothermal_microcalorimetry wikiPageWikiLink Macrophage.
- Isothermal_microcalorimetry wikiPageWikiLink Macrophages.
- Isothermal_microcalorimetry wikiPageWikiLink Malic_acid.
- Isothermal_microcalorimetry wikiPageWikiLink Metabolomics.
- Isothermal_microcalorimetry wikiPageWikiLink Moonmilk.
- Isothermal_microcalorimetry wikiPageWikiLink Peltier-Seebeck_effect.
- Isothermal_microcalorimetry wikiPageWikiLink Platelet.
- Isothermal_microcalorimetry wikiPageWikiLink Platelets.
- Isothermal_microcalorimetry wikiPageWikiLink Rate_equation.
- Isothermal_microcalorimetry wikiPageWikiLink Red_blood_cell.
- Isothermal_microcalorimetry wikiPageWikiLink Thermal_analysis.
- Isothermal_microcalorimetry wikiPageWikiLink Thermoelectric_effect.
- Isothermal_microcalorimetry wikiPageWikiLink Tissue_engineering.
- Isothermal_microcalorimetry wikiPageWikiLink Tuberculosis.
- Isothermal_microcalorimetry wikiPageWikiLink File:An_artists_rendition_of_an_isothermal_microcalorimetry_(IMC)_instrument_and_one_of_its_measurement_units.jpg.
- Isothermal_microcalorimetry wikiPageWikiLink File:Glass_ampoule_for_isothermal_microcalorimetry.png.
- Isothermal_microcalorimetry wikiPageWikiLink File:IMC_Daniels_02.jpg.
- Isothermal_microcalorimetry wikiPageWikiLink File:IMC_Daniels_06.jpg.
- Isothermal_microcalorimetry wikiPageWikiLink File:IMC_Daniels_07.jpg.
- Isothermal_microcalorimetry wikiPageWikiLinkText "Isothermal microcalorimetry".
- Isothermal_microcalorimetry hasPhotoCollection Isothermal_microcalorimetry.
- Isothermal_microcalorimetry wikiPageUsesTemplate Template:Cite_journal.
- Isothermal_microcalorimetry wikiPageUsesTemplate Template:Fact.
- Isothermal_microcalorimetry wikiPageUsesTemplate Template:Infobox_chemical_analysis.
- Isothermal_microcalorimetry wikiPageUsesTemplate Template:Reflist.
- Isothermal_microcalorimetry subject Category:Biological_processes.
- Isothermal_microcalorimetry subject Category:Calorimetry.
- Isothermal_microcalorimetry subject Category:Chemical_processes.
- Isothermal_microcalorimetry subject Category:Heat_transfer.
- Isothermal_microcalorimetry hypernym Method.
- Isothermal_microcalorimetry type Article.
- Isothermal_microcalorimetry type Software.
- Isothermal_microcalorimetry type Article.
- Isothermal_microcalorimetry type Process.
- Isothermal_microcalorimetry type Thermodynamic.
- Isothermal_microcalorimetry comment "Isothermal microcalorimetry (IMC) is a laboratory method for real-time monitoring and dynamic analysis of chemical, physical and biological processes. Over a period of hours or days, IMC determines the onset, rate, extent and energetics of such processes for specimens in small ampoules (e.g. 3–20 ml) at a constant set temperature (ca. 15°C–150°C).IMC accomplishes this dynamic analysis by measuring and recording vs.".
- Isothermal_microcalorimetry label "Isothermal microcalorimetry".
- Isothermal_microcalorimetry sameAs m.0jt1jg1.
- Isothermal_microcalorimetry sameAs Q6086400.
- Isothermal_microcalorimetry sameAs Q6086400.
- Isothermal_microcalorimetry wasDerivedFrom Isothermal_microcalorimetry?oldid=674049784.
- Isothermal_microcalorimetry depiction An_artists_rendition_of_an_isothermal_microcalorimetry_(IMC)_instrument_and_one_of_its_measurement_units.jpg.
- Isothermal_microcalorimetry isPrimaryTopicOf Isothermal_microcalorimetry.