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- Helper_dependent_virus abstract "A helper dependent virus also termed a gutless virus is a synthetic viral vector dependent on the assistance of a helper virus in order to replicate.Since the genome of the gutless virus does not include genes encoding the enzymes and/or structural proteins required to replicate, it is deemed safe for use in gene therapy since an infection cannot occur except in the presence of a suitable helper virus.Well established protocols allow scientists to propagate helper dependent viruses in the lab, however, using an actual helper virus poses problems when it comes to purification of desired transgenic virus. Therefore lab methods often utilize minimal fragments of the helper DNA that can serve this purpose without creating unwanted virus. This process usually involves the introduction of three separate DNA plasmids into a eukaryotic cell line through a process called transfection. These plasmids contain either transgenic DNA, replication and capsid encoding DNA, and helper DNA. Every cell that is successfully transfected with all three DNA fragments will produce the necessary proteins to produce infective viruses. These viruses will only have transgenic DNA encapsidated and therefore once they've infected a patient's cell, they will not be capable of reproducing.Helper dependent viruses can also occur in nature without being "gutted." The term satellite virus has been given to a large group of viruses that all require presence of another virus to replicate. Many of these are plant viruses, but animal viruses can be seen in the cases of dependoviruses. Within the family parvoviridae, the dependovirus genus was given a distinct classification due to their dependence on another virus. The most widely known dependovirus is adeno-associated virus(AAV) which was originally discovered as a contaminant of a sample of simian adenovirus. Though AAV is considered to be dependent on adenovirus, it is able to replicate in the presence of herpesvirus as well as certain cytotoxic events such as UV irradiation or some carcinogens During the course of a natural dependovirus infection, if the helper virus is not present, the dependovirus is often capable of integrating into the host genome and going into a latent phase of its life cycle—effectively waiting for the next helper virus infection. For gene therapy uses, the vector is stripped of its ability to integrate. Because AAV can deliver transgenic material in a non-replicating form, it is a strong candidate for gene therapy and is currently used in about 5% of clinical trials.".
- Helper_dependent_virus wikiPageID "6478160".
- Helper_dependent_virus wikiPageLength "4418".
- Helper_dependent_virus wikiPageOutDegree "21".
- Helper_dependent_virus wikiPageRevisionID "638183514".
- Helper_dependent_virus wikiPageWikiLink Adeno-associated_virus.
- Helper_dependent_virus wikiPageWikiLink Adenoviridae.
- Helper_dependent_virus wikiPageWikiLink Adenovirus.
- Helper_dependent_virus wikiPageWikiLink Capsid.
- Helper_dependent_virus wikiPageWikiLink Carcinogen.
- Helper_dependent_virus wikiPageWikiLink Category:Virology.
- Helper_dependent_virus wikiPageWikiLink Dependoparvovirus.
- Helper_dependent_virus wikiPageWikiLink Dependovirus.
- Helper_dependent_virus wikiPageWikiLink Eukaryote.
- Helper_dependent_virus wikiPageWikiLink Eukaryotic.
- Helper_dependent_virus wikiPageWikiLink Gene_therapy.
- Helper_dependent_virus wikiPageWikiLink Helper_virus.
- Helper_dependent_virus wikiPageWikiLink Herpesviridae.
- Helper_dependent_virus wikiPageWikiLink Herpesvirus.
- Helper_dependent_virus wikiPageWikiLink Parvoviridae.
- Helper_dependent_virus wikiPageWikiLink Plasmid.
- Helper_dependent_virus wikiPageWikiLink Satellite_(biology).
- Helper_dependent_virus wikiPageWikiLink Satellite_virus.
- Helper_dependent_virus wikiPageWikiLink Simian.
- Helper_dependent_virus wikiPageWikiLink Site-specific_recombinase_technology.
- Helper_dependent_virus wikiPageWikiLink Transfection.
- Helper_dependent_virus wikiPageWikiLink Transgenesis.
- Helper_dependent_virus wikiPageWikiLink Transgenic.
- Helper_dependent_virus wikiPageWikiLink UV.
- Helper_dependent_virus wikiPageWikiLink Ultraviolet.
- Helper_dependent_virus wikiPageWikiLink Viral_replication.
- Helper_dependent_virus wikiPageWikiLink Viral_vector.
- Helper_dependent_virus wikiPageWikiLink Virus_latency.
- Helper_dependent_virus wikiPageWikiLinkText "Helper dependent virus".
- Helper_dependent_virus wikiPageWikiLinkText "helper dependent viral vector".
- Helper_dependent_virus hasPhotoCollection Helper_dependent_virus.
- Helper_dependent_virus wikiPageUsesTemplate Template:Reflist.
- Helper_dependent_virus subject Category:Virology.
- Helper_dependent_virus hypernym Vector.
- Helper_dependent_virus type Article.
- Helper_dependent_virus type Insect.
- Helper_dependent_virus type Article.
- Helper_dependent_virus comment "A helper dependent virus also termed a gutless virus is a synthetic viral vector dependent on the assistance of a helper virus in order to replicate.Since the genome of the gutless virus does not include genes encoding the enzymes and/or structural proteins required to replicate, it is deemed safe for use in gene therapy since an infection cannot occur except in the presence of a suitable helper virus.Well established protocols allow scientists to propagate helper dependent viruses in the lab, however, using an actual helper virus poses problems when it comes to purification of desired transgenic virus. ".
- Helper_dependent_virus label "Helper dependent virus".
- Helper_dependent_virus sameAs فيروس_مساعد_مستقل.
- Helper_dependent_virus sameAs m.0g6tvp.
- Helper_dependent_virus sameAs Q5710212.
- Helper_dependent_virus sameAs Q5710212.
- Helper_dependent_virus wasDerivedFrom Helper_dependent_virus?oldid=638183514.
- Helper_dependent_virus isPrimaryTopicOf Helper_dependent_virus.