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- HELP_assay abstract "The HpaII tiny fragment Enrichment by Ligation-mediated PCR Assay (HELP Assay) is one of several techniques used for determining whether DNA has been methylated. The technique can be adapted to examine DNA methylation within and around individual genes, or it can be expanded to examine methylation in an entire genome.The technique relies upon the properties of two restriction enzymes: HpaII and MspI. The HELP assay compares representations generated by HpaII and by MspI digestion of the genome followed by ligation-mediated PCR. HpaII only digests 5'-CCGG-3' sites when the cytosine in the central CG dinucleotide is unmethylated, the HpaII representation is enriched for the hypomethylated fraction of the genome. The MspI representation is a control for copy number changes and PCR amplification difficulties.It was recently shown that cytosine methylation patterns tend to be concordant over short (~1 kb) regions. The patterns represented by the HpaII sites therefore tend to be representative of other CG dinucleotides locally.The analysis of HELP data involves quality analysis and normalization. An analytical pipeline written in the R programming language was recently published to allow HELP data processing.".
- HELP_assay wikiPageExternalLink help.html.
- HELP_assay wikiPageID "8855086".
- HELP_assay wikiPageLength "2503".
- HELP_assay wikiPageOutDegree "8".
- HELP_assay wikiPageRevisionID "678842139".
- HELP_assay wikiPageWikiLink Category:Microbiology.
- HELP_assay wikiPageWikiLink DNA_methylation.
- HELP_assay wikiPageWikiLink Gene.
- HELP_assay wikiPageWikiLink Genes.
- HELP_assay wikiPageWikiLink Genome.
- HELP_assay wikiPageWikiLink PCR.
- HELP_assay wikiPageWikiLink Polymerase_chain_reaction.
- HELP_assay wikiPageWikiLink R_(programming_language).
- HELP_assay wikiPageWikiLink R_programming_language.
- HELP_assay wikiPageWikiLink Restriction_enzyme.
- HELP_assay wikiPageWikiLink Restriction_enzymes.
- HELP_assay wikiPageWikiLinkText "HELP assay".
- HELP_assay hasPhotoCollection HELP_assay.
- HELP_assay wikiPageUsesTemplate Template:Context.
- HELP_assay wikiPageUsesTemplate Template:Reflist.
- HELP_assay subject Category:Microbiology.
- HELP_assay hypernym Techniques.
- HELP_assay type Article.
- HELP_assay type Weapon.
- HELP_assay type Article.
- HELP_assay type Page.
- HELP_assay comment "The HpaII tiny fragment Enrichment by Ligation-mediated PCR Assay (HELP Assay) is one of several techniques used for determining whether DNA has been methylated. The technique can be adapted to examine DNA methylation within and around individual genes, or it can be expanded to examine methylation in an entire genome.The technique relies upon the properties of two restriction enzymes: HpaII and MspI.".
- HELP_assay label "HELP assay".
- HELP_assay sameAs مقايسة_HELP.
- HELP_assay sameAs m.027m4dc.
- HELP_assay sameAs Q5629446.
- HELP_assay sameAs Q5629446.
- HELP_assay wasDerivedFrom HELP_assay?oldid=678842139.
- HELP_assay isPrimaryTopicOf HELP_assay.