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- Guanidinium_thiocyanate abstract "Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.Note: this compound may also be recognized as guanidine thiocyanate. This is because guanidinium is the conjugate acid of guanidine and is called the guanidinium cation, [CH6N3]+.Guanidinium thiocyanate can be used to deactivate a virus, such as the influenza virus that caused the 1918 "Spanish flu", so that it can be studied safely. This application is presumably based on its denaturation activity.Guanidinium thiocyanate is also used to lyse cells and virus particles in RNA and DNA extractions, where its function, in addition to its lysing action, is to prevent activity of RNase enzymes and DNase enzymes by denaturing them. These enzymes would otherwise damage the extract. A commonly used method is guanidinium thiocyanate-phenol-chloroform extraction. It is not strictly necessary to use phenol or chloroform if extracting RNA for Northern blotting or DNA for Southern blot analysis because the gel electrophoresis followed by transfer to a membrane will separate the RNA/DNA from the proteins. Additionally, since these methods use probes to bind to their conjugates, peptides that get through the process don't generally matter unless a peptide is an RNase or DNase, and then only if the enzyme manages to renature, which should not occur if proper protocols are followed. A possible exception might be when working with temperature extremophiles because some enzymes of these organisms can remain stable under extraordinary circumstances.".
- Guanidinium_thiocyanate thumbnail Guanidinium_thiocyanate.svg?width=300.
- Guanidinium_thiocyanate wikiPageExternalLink 01933.htm.
- Guanidinium_thiocyanate wikiPageID "2870145".
- Guanidinium_thiocyanate wikiPageLength "3350".
- Guanidinium_thiocyanate wikiPageOutDegree "12".
- Guanidinium_thiocyanate wikiPageRevisionID "642309280".
- Guanidinium_thiocyanate wikiPageWikiLink 1918_flu_pandemic.
- Guanidinium_thiocyanate wikiPageWikiLink Acid_guanidinium_thiocyanate-phenol-chloroform_extraction.
- Guanidinium_thiocyanate wikiPageWikiLink Category:Guanidines.
- Guanidinium_thiocyanate wikiPageWikiLink Category:Thiocyanates.
- Guanidinium_thiocyanate wikiPageWikiLink Chaotropic_agent.
- Guanidinium_thiocyanate wikiPageWikiLink Chemical_compound.
- Guanidinium_thiocyanate wikiPageWikiLink Guanidine.
- Guanidinium_thiocyanate wikiPageWikiLink Guanidinium_cation.
- Guanidinium_thiocyanate wikiPageWikiLink Guanidinium_thiocyanate-phenol-chloroform_extraction.
- Guanidinium_thiocyanate wikiPageWikiLink Influenza.
- Guanidinium_thiocyanate wikiPageWikiLink Lysis.
- Guanidinium_thiocyanate wikiPageWikiLink Northern_blot.
- Guanidinium_thiocyanate wikiPageWikiLink Spanish_flu.
- Guanidinium_thiocyanate wikiPageWikiLink Virus.
- Guanidinium_thiocyanate wikiPageWikiLinkText "Guanidinium thiocyanate".
- Guanidinium_thiocyanate wikiPageWikiLinkText "guanidinium thiocyanate".
- Guanidinium_thiocyanate hasPhotoCollection Guanidinium_thiocyanate.
- Guanidinium_thiocyanate imagefile "Guanidinium thiocyanate.svg".
- Guanidinium_thiocyanate verifiedrevid "449114769".
- Guanidinium_thiocyanate wikiPageUsesTemplate Template:Biochem-stub.
- Guanidinium_thiocyanate wikiPageUsesTemplate Template:Chembox.
- Guanidinium_thiocyanate wikiPageUsesTemplate Template:Organic-compound-stub.
- Guanidinium_thiocyanate subject Category:Guanidines.
- Guanidinium_thiocyanate subject Category:Thiocyanates.
- Guanidinium_thiocyanate hypernym Compound.
- Guanidinium_thiocyanate type ChemicalCompound.
- Guanidinium_thiocyanate type ChemicalSubstance.
- Guanidinium_thiocyanate type Group.
- Guanidinium_thiocyanate type Group.
- Guanidinium_thiocyanate type ChemicalObject.
- Guanidinium_thiocyanate type Thing.
- Guanidinium_thiocyanate type Q11173.
- Guanidinium_thiocyanate comment "Guanidinium thiocyanate (GITC) is a chemical compound used as a general protein denaturant, being a chaotropic agent, although it is most commonly used in the extraction of DNA and RNA.Note: this compound may also be recognized as guanidine thiocyanate.".
- Guanidinium_thiocyanate label "Guanidinium thiocyanate".
- Guanidinium_thiocyanate sameAs Guanidiniumthiocyanat.
- Guanidinium_thiocyanate sameAs Tiocianato_de_guanidinio.
- Guanidinium_thiocyanate sameAs گوآنیدینیوم_تیوسیانات.
- Guanidinium_thiocyanate sameAs Thiocyanate_de_guanidinium.
- Guanidinium_thiocyanate sameAs m.0881dz.
- Guanidinium_thiocyanate sameAs Gvanidinijum_tiocijanat.
- Guanidinium_thiocyanate sameAs Gvanidinijum_tiocijanat.
- Guanidinium_thiocyanate sameAs Q904487.
- Guanidinium_thiocyanate sameAs Q904487.
- Guanidinium_thiocyanate wasDerivedFrom Guanidinium_thiocyanate?oldid=642309280.
- Guanidinium_thiocyanate depiction Guanidinium_thiocyanate.svg.
- Guanidinium_thiocyanate isPrimaryTopicOf Guanidinium_thiocyanate.