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- Fluo-3 abstract "Fluo-3 is a fluorescence indicator of intracellular calcium (Ca2+). It is used to measure Ca2+ inside living cells in flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluo-3 is an essentially nonfluorescent compound, but upon binding of Ca2+ its fluorescence increases sharply with an emission maximum at 525 nm suitable for conventionally used detectors designed for fluorescein isothiocyanate (FITC) measurements. This large change in fluorescence coupled with a good yield of photons provides very high contrast which allowed the detection of microscopic Ca2+ release events inside cells called "Calcium sparks". Whereas the salts of fluo-3 are unable to penetrate cells, loading can be achieved using its acetoxymethyl (AM) ester derivative. Once inside the cell, unspecific esterases cleave the ester effectively trapping fluo-3.As calcium is a key second messenger within cells, the specific properties of fluo-3 enable researchers to investigate the time-resolved dynamics of intracellular signal transduction in a diverse range of cells.".
- Fluo-3 iupacName "{[2-(2-{2-[Bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy}ethoxy)-4-methylphenyl](carboxymethyl)amino}acetic acid".
- Fluo-3 thumbnail Fluo-3.svg?width=300.
- Fluo-3 wikiPageID "36436992".
- Fluo-3 wikiPageLength "3273".
- Fluo-3 wikiPageOutDegree "13".
- Fluo-3 wikiPageRevisionID "670415583".
- Fluo-3 wikiPageWikiLink Argon_laser.
- Fluo-3 wikiPageWikiLink Calcium_in_biology.
- Fluo-3 wikiPageWikiLink Calcium_sparks.
- Fluo-3 wikiPageWikiLink Category:Cell_imaging.
- Fluo-3 wikiPageWikiLink Category:Fluorescent_dyes.
- Fluo-3 wikiPageWikiLink Confocal_laser_scanning_microscopy.
- Fluo-3 wikiPageWikiLink Ester.
- Fluo-3 wikiPageWikiLink Esterase.
- Fluo-3 wikiPageWikiLink Flow_cytometry.
- Fluo-3 wikiPageWikiLink Fluorescein_isothiocyanate.
- Fluo-3 wikiPageWikiLink Fluorescence.
- Fluo-3 wikiPageWikiLink Ion_laser.
- Fluo-3 wikiPageWikiLink Second_messenger.
- Fluo-3 wikiPageWikiLink Second_messenger_system.
- Fluo-3 wikiPageWikiLink Signal_transduction.
- Fluo-3 wikiPageWikiLinkText "Fluo-3".
- Fluo-3 wikiPageWikiLinkText "fluo-3".
- Fluo-3 hasPhotoCollection Fluo-3.
- Fluo-3 imagefile "Fluo-3.svg".
- Fluo-3 imagesize "200".
- Fluo-3 iupacname "{[2--4-methylphenyl]amino}acetic acid".
- Fluo-3 wikiPageUsesTemplate Template:Chembox.
- Fluo-3 wikiPageUsesTemplate Template:Reflist.
- Fluo-3 subject Category:Cell_imaging.
- Fluo-3 subject Category:Fluorescent_dyes.
- Fluo-3 hypernym Indicator.
- Fluo-3 type ChemicalCompound.
- Fluo-3 type ChemicalSubstance.
- Fluo-3 type Technique.
- Fluo-3 type ChemicalObject.
- Fluo-3 type Thing.
- Fluo-3 type Q11173.
- Fluo-3 comment "Fluo-3 is a fluorescence indicator of intracellular calcium (Ca2+). It is used to measure Ca2+ inside living cells in flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm).".
- Fluo-3 label "Fluo-3".
- Fluo-3 sameAs m.0k8zgwx.
- Fluo-3 sameAs Fluo-3.
- Fluo-3 sameAs Fluo-3.
- Fluo-3 sameAs Q8564077.
- Fluo-3 sameAs Q8564077.
- Fluo-3 wasDerivedFrom Fluo-3?oldid=670415583.
- Fluo-3 depiction Fluo-3.svg.
- Fluo-3 isPrimaryTopicOf Fluo-3.