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- Q5397193 subject Q8680982.
- Q5397193 abstract "Escherichia coli contains a number of small RNAs located in intergenic regions of its genome. The presence of at least 55 of these has been verified experimentally. 275 potential sRNA-encoding loci were identified computationally using the QRNA program. These loci will include false positives, so the number of sRNA genes in E. coli is likely to be less than 275. A computational screen based on promoter sequences recognised by the sigma factor sigma 70 and on Rho-independent terminators predicted 24 putative sRNA genes, 14 of these were verified experimentally by northern blotting. The experimentally verified sRNAs included the well characterised sRNAs RprA and RyhB. Many of the sRNAs identified in this screen, including RprA, RyhB, SraB and SraL, are only expressed in the stationary phase of bacterial cell growth. A screen for sRNA genes based on homology to Salmonella and Klebsiella identified 59 candidate sRNA genes. From this set of candidate genes, microarray analysis and northern blotting confirmed the existence of 17 previously undescribed sRNAs, many of which bind to the chaperone protein Hfq and regulate the translation of RpoS (Sigma 38).".
- Q5397193 thumbnail SraA_secondary_structure.jpg?width=300.
- Q5397193 wikiPageExternalLink rfam.sanger.ac.uk.
- Q5397193 wikiPageExternalLink RF02029.
- Q5397193 wikiPageExternalLink RF02030.
- Q5397193 wikiPageExternalLink RF02031.
- Q5397193 wikiPageWikiLink Q106227.
- Q5397193 wikiPageWikiLink Q131854.
- Q5397193 wikiPageWikiLink Q150839.
- Q5397193 wikiPageWikiLink Q17148058.
- Q5397193 wikiPageWikiLink Q1931516.
- Q5397193 wikiPageWikiLink Q224093.
- Q5397193 wikiPageWikiLink Q224180.
- Q5397193 wikiPageWikiLink Q25419.
- Q5397193 wikiPageWikiLink Q306436.
- Q5397193 wikiPageWikiLink Q307446.
- Q5397193 wikiPageWikiLink Q3463539.
- Q5397193 wikiPageWikiLink Q3822799.
- Q5397193 wikiPageWikiLink Q422496.
- Q5397193 wikiPageWikiLink Q4838982.
- Q5397193 wikiPageWikiLink Q48520.
- Q5397193 wikiPageWikiLink Q5016837.
- Q5397193 wikiPageWikiLink Q6595358.
- Q5397193 wikiPageWikiLink Q6947108.
- Q5397193 wikiPageWikiLink Q7020.
- Q5397193 wikiPageWikiLink Q7255106.
- Q5397193 wikiPageWikiLink Q7375543.
- Q5397193 wikiPageWikiLink Q7375545.
- Q5397193 wikiPageWikiLink Q7385058.
- Q5397193 wikiPageWikiLink Q7582371.
- Q5397193 wikiPageWikiLink Q7702794.
- Q5397193 wikiPageWikiLink Q8680982.
- Q5397193 comment "Escherichia coli contains a number of small RNAs located in intergenic regions of its genome. The presence of at least 55 of these has been verified experimentally. 275 potential sRNA-encoding loci were identified computationally using the QRNA program. These loci will include false positives, so the number of sRNA genes in E. coli is likely to be less than 275.".
- Q5397193 label "Escherichia coli sRNA".
- Q5397193 depiction SraA_secondary_structure.jpg.