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- Q5090513 subject Q6967682.
- Q5090513 subject Q7214254.
- Q5090513 subject Q7217012.
- Q5090513 subject Q7305817.
- Q5090513 abstract "A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.There is a clear distinction between serum-free media and chemically defined media. Serum-free media may contain undefined animal-derived products such as serum albumin (purified from blood), hydrolysates, growth factors, hormones, carrier proteins, and attachment factors. These undefined animal-derived products will contain complex contaminants, such as the lipid content of albumin. In contrast, chemically defined media require that all of the components must be identified and have their exact concentrations known. Therefore, a chemically defined medium must be entirely free of animal-derived components and cannot contain either fetal bovine serum, bovine serum albumin or human serum albumin. To achieve this chemically defined media is commonly supplemented with recombinant versions of albumin and growth factors, usually derived from rice or E. coli, or synthetic chemical such as the polymer polyvinyl alcohol which can reproduce some of the functions of BSA/HSA.The constituents of a chemically defined media include: a basal media (such as DMEM, F12, or RPMI 1640, containing amino acids, vitamins, inorganic salts, buffers, antioxidants and energy sources), which is supplemented with recombinant albumin, chemically defined lipids, recombinant insulin and/or zinc, recombinant transferrin or iron, selenium and an antioxidant thiol such as 2-mercaptoethanol or 1-thioglycerol.".
- Q5090513 wikiPageExternalLink products.
- Q5090513 wikiPageExternalLink 332-032442_SFMBrochure.pdf.
- Q5090513 wikiPageExternalLink PerspectivesWEB.pdf.
- Q5090513 wikiPageExternalLink page_13875.
- Q5090513 wikiPageWikiLink Q11721976.
- Q5090513 wikiPageWikiLink Q146339.
- Q5090513 wikiPageWikiLink Q14860550.
- Q5090513 wikiPageWikiLink Q1549003.
- Q5090513 wikiPageWikiLink Q1581434.
- Q5090513 wikiPageWikiLink Q1660153.
- Q5090513 wikiPageWikiLink Q189082.
- Q5090513 wikiPageWikiLink Q21163221.
- Q5090513 wikiPageWikiLink Q220410.
- Q5090513 wikiPageWikiLink Q221681.
- Q5090513 wikiPageWikiLink Q2620381.
- Q5090513 wikiPageWikiLink Q2662727.
- Q5090513 wikiPageWikiLink Q2745755.
- Q5090513 wikiPageWikiLink Q3007160.
- Q5090513 wikiPageWikiLink Q3287622.
- Q5090513 wikiPageWikiLink Q3634161.
- Q5090513 wikiPageWikiLink Q411084.
- Q5090513 wikiPageWikiLink Q488614.
- Q5090513 wikiPageWikiLink Q5058183.
- Q5090513 wikiPageWikiLink Q575920.
- Q5090513 wikiPageWikiLink Q6967682.
- Q5090513 wikiPageWikiLink Q7214254.
- Q5090513 wikiPageWikiLink Q7217012.
- Q5090513 wikiPageWikiLink Q7305817.
- Q5090513 wikiPageWikiLink Q7868.
- Q5090513 wikiPageWikiLink Q876.
- Q5090513 comment "A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. Standard cell culture media are commonly supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors.".
- Q5090513 label "Chemically defined medium".