Matches in DBpedia 2016-04 for { <http://wikidata.dbpedia.org/resource/Q5013338> ?p ?o }
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- Q5013338 subject Q6967682.
- Q5013338 subject Q7164711.
- Q5013338 subject Q7214254.
- Q5013338 subject Q7400651.
- Q5013338 subject Q8358944.
- Q5013338 subject Q8579496.
- Q5013338 abstract "COLD-PCR (co-amplification at lower denaturation temperature-PCR) is a modified Polymerase Chain Reaction (PCR) protocol that enriches variant alleles from a mixture of wildtype and mutation-containing DNA. The ability to preferentially amplify and identify minority alleles and low-level somatic DNA mutations in the presence of excess wildtype alleles is useful for the detection of mutations. Detection of mutations is important in the case of early cancer detection from tissue biopsies and body fluids such as blood plasma or serum, assessment of residual disease after surgery or chemotherapy, disease staging and molecular profiling for prognosis or tailoring therapy to individual patients, and monitoring of therapy outcome and cancer remission or relapse. Common PCR will amplify both the major (wildtype) and minor (mutant) alleles with the same efficiency, occluding the ability to easily detect the presence of low-level mutations. The capacity to detect a mutation in a mixture of variant/wildtype DNA is valuable because this mixture of variant DNAs can occur when provided with a heterogeneous sample – as is often the case with cancer biopsies. Currently, traditional PCR is used in tandem with a number of different downstream assays for genotyping or the detection of somatic mutations. These can include the use of amplified DNA for RFLP analysis, MALDI-TOF (matrix-assisted laser-desorption–time-of-flight) genotyping, or direct sequencing for detection of mutations by Sanger sequencing or pyrosequencing. Replacing traditional PCR with COLD-PCR for these downstream assays will increase the reliability in detecting mutations from mixed samples, including tumors and body fluids.".
- Q5013338 thumbnail COLD-PCR.jpg?width=300.
- Q5013338 wikiPageWikiLink Q110084.
- Q5013338 wikiPageWikiLink Q12078.
- Q5013338 wikiPageWikiLink Q12136.
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- Q5013338 wikiPageWikiLink Q14818098.
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- Q5013338 wikiPageWikiLink Q2342248.
- Q5013338 wikiPageWikiLink Q28745.
- Q5013338 wikiPageWikiLink Q356033.
- Q5013338 wikiPageWikiLink Q40397.
- Q5013338 wikiPageWikiLink Q40821.
- Q5013338 wikiPageWikiLink Q42918.
- Q5013338 wikiPageWikiLink Q501128.
- Q5013338 wikiPageWikiLink Q6967682.
- Q5013338 wikiPageWikiLink Q7164711.
- Q5013338 wikiPageWikiLink Q7214254.
- Q5013338 wikiPageWikiLink Q736400.
- Q5013338 wikiPageWikiLink Q7391886.
- Q5013338 wikiPageWikiLink Q7400651.
- Q5013338 wikiPageWikiLink Q7430.
- Q5013338 wikiPageWikiLink Q7684883.
- Q5013338 wikiPageWikiLink Q79749.
- Q5013338 wikiPageWikiLink Q80726.
- Q5013338 wikiPageWikiLink Q8358944.
- Q5013338 wikiPageWikiLink Q856198.
- Q5013338 wikiPageWikiLink Q857299.
- Q5013338 wikiPageWikiLink Q8579496.
- Q5013338 wikiPageWikiLink Q912147.
- Q5013338 wikiPageWikiLink Q974135.
- Q5013338 comment "COLD-PCR (co-amplification at lower denaturation temperature-PCR) is a modified Polymerase Chain Reaction (PCR) protocol that enriches variant alleles from a mixture of wildtype and mutation-containing DNA. The ability to preferentially amplify and identify minority alleles and low-level somatic DNA mutations in the presence of excess wildtype alleles is useful for the detection of mutations.".
- Q5013338 label "COLD-PCR".
- Q5013338 depiction COLD-PCR.jpg.