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- Q4839995 subject Q4926419.
- Q4839995 abstract "The bacterial one-hybrid (B1H) system is a method for identifying the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences are cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence.".
- Q4839995 thumbnail FigureB1H.JPG?width=300.
- Q4839995 wikiPageExternalLink B1HStart.pdf.
- Q4839995 wikiPageExternalLink wolfe.cfm?start=0&.
- Q4839995 wikiPageWikiLink Q1093165.
- Q4839995 wikiPageWikiLink Q1337844.
- Q4839995 wikiPageWikiLink Q13479514.
- Q4839995 wikiPageWikiLink Q172778.
- Q4839995 wikiPageWikiLink Q177900.
- Q4839995 wikiPageWikiLink Q1931516.
- Q4839995 wikiPageWikiLink Q204785.
- Q4839995 wikiPageWikiLink Q224093.
- Q4839995 wikiPageWikiLink Q25419.
- Q4839995 wikiPageWikiLink Q272631.
- Q4839995 wikiPageWikiLink Q407384.
- Q4839995 wikiPageWikiLink Q411391.
- Q4839995 wikiPageWikiLink Q461499.
- Q4839995 wikiPageWikiLink Q4926419.
- Q4839995 wikiPageWikiLink Q5629690.
- Q4839995 wikiPageWikiLink Q7815764.
- Q4839995 wikiPageWikiLink Q7865588.
- Q4839995 wikiPageWikiLink Q851162.
- Q4839995 wikiPageWikiLink Q896177.
- Q4839995 wikiPageWikiLink Q907101.
- Q4839995 wikiPageWikiLink Q911828.
- Q4839995 comment "The bacterial one-hybrid (B1H) system is a method for identifying the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences are cloned into a separate vector containing the selectable genes HIS3 and URA3.".
- Q4839995 label "Bacterial one-hybrid system".
- Q4839995 depiction FigureB1H.JPG.