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- Q415601 subject Q6368047.
- Q415601 abstract "Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. Some, such as Deoxyribonuclease I, cut DNA relatively nonspecifically (without regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences.Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence. The nucleotide sequence recognized for cleavage by a restriction enzyme is called the restriction site. Typically, a restriction site will be a palindromic sequence of about four to six nucleotides long. Most restriction endonucleases cleave the DNA strand unevenly, leaving complementary single-stranded ends. These ends can reconnect through hybridization and are termed "sticky ends". Once paired, the phosphodiester bonds of the fragments can be joined by DNA ligase. There are hundreds of restriction endonucleases known, each attacking a different restriction site. The DNA fragments cleaved by the same endonuclease can be joined together regardless of the origin of the DNA. Such DNA is called recombinant DNA; DNA formed by the joining of genes into new combinations. Restriction endonucleases (restriction enzymes) are divided into three categories, Type I, Type II, and Type III, according to their mechanism of action. These enzymes are often used in genetic engineering to make recombinant DNA for introduction into bacterial, plant, or animal cells, as well as in synthetic biology.".
- Q415601 thumbnail Restriction_enzyme_Eco_RI.JPG?width=300.
- Q415601 wikiPageWikiLink Q12321.
- Q415601 wikiPageWikiLink Q14901483.
- Q415601 wikiPageWikiLink Q159236.
- Q415601 wikiPageWikiLink Q1624837.
- Q415601 wikiPageWikiLink Q180618.
- Q415601 wikiPageWikiLink Q219715.
- Q415601 wikiPageWikiLink Q285697.
- Q415601 wikiPageWikiLink Q408483.
- Q415601 wikiPageWikiLink Q411641.
- Q415601 wikiPageWikiLink Q411664.
- Q415601 wikiPageWikiLink Q415601.
- Q415601 wikiPageWikiLink Q415875.
- Q415601 wikiPageWikiLink Q422523.
- Q415601 wikiPageWikiLink Q422529.
- Q415601 wikiPageWikiLink Q4653506.
- Q415601 wikiPageWikiLink Q471857.
- Q415601 wikiPageWikiLink Q612693.
- Q415601 wikiPageWikiLink Q6368047.
- Q415601 wikiPageWikiLink Q648135.
- Q415601 wikiPageWikiLink Q8047.
- Q415601 wikiPageWikiLink Q80756.
- Q415601 wikiPageWikiLink Q862838.
- Q415601 wikiPageWikiLink Q863983.
- Q415601 comment "Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. Some, such as Deoxyribonuclease I, cut DNA relatively nonspecifically (without regard to sequence), while many, typically called restriction endonucleases or restriction enzymes, cleave only at very specific nucleotide sequences.Restriction enzymes are endonucleases from eubacteria and archaea that recognize a specific DNA sequence.".
- Q415601 label "Endonuclease".
- Q415601 depiction Restriction_enzyme_Eco_RI.JPG.