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- Q3543449 subject Q7214254.
- Q3543449 subject Q8266666.
- Q3543449 subject Q8482020.
- Q3543449 abstract "Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a living cell but may also include intercellular space. Microinjection is a simple mechanical process usually involving an inverted microscope with a magnification power of around 200x (though sometimes it is performed using a dissecting stereo microscope at 40–50x or a traditional compound upright microscope at similar power to an inverted model).For processes such as cellular or pronuclear injection the target cell is positioned under the microscope and two micromanipulators—one holding the pipette and one holding a microcapillary needle usually between 0.5 to 5 µm in diameter (larger if injecting stem cells into an embryo)—are used to penetrate the cell membrane and/or the nuclear envelope. In this way the process can be used to introduce a vector into a single cell. Microinjection can also be used in the cloning of organisms, in the study of cell biology and viruses, and for treating male subfertility through intracytoplasmic sperm injection (ICSI, "IK-see").".
- Q3543449 thumbnail Microinjector_microinjection_controller.jpg?width=300.
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- Q3543449 wikiPageWikiLink Q7214254.
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- Q3543449 wikiPageWikiLink Q8266666.
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- Q3543449 wikiPageWikiLink Q8482020.
- Q3543449 wikiPageWikiLink Q912094.
- Q3543449 wikiPageWikiLink Q912313.
- Q3543449 comment "Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a living cell but may also include intercellular space.".
- Q3543449 label "Microinjection".
- Q3543449 depiction Microinjector_microinjection_controller.jpg.