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- Q2124139 subject Q7029056.
- Q2124139 abstract "RESOLFT, an acronym for REversible Saturable OpticaL Fluorescence Transitions, denotes a group of optical microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained.With conventional microscopy techniques, it is not possible to distinguish features that are located at distances less than about half the wavelength used (i.e. about 200 nm for visible light). This diffraction limit is based on the wave nature of light. In conventional microscopes the limit is determined by the used wavelength and the numerical aperture of the optical system. The RESOLFT concept surmounts this limit by temporarily switching the molecules to a state in which they cannot send a (fluorescence-) signal upon illumination. This concept is different from for example electron microscopy where instead the used wavelength is much smaller.".
- Q2124139 thumbnail RESOLFT_principle.jpg?width=300.
- Q2124139 wikiPageWikiLink Q1074953.
- Q2124139 wikiPageWikiLink Q132560.
- Q2124139 wikiPageWikiLink Q13405516.
- Q2124139 wikiPageWikiLink Q238165.
- Q2124139 wikiPageWikiLink Q272983.
- Q2124139 wikiPageWikiLink Q3707396.
- Q2124139 wikiPageWikiLink Q41364.
- Q2124139 wikiPageWikiLink Q467358.
- Q2124139 wikiPageWikiLink Q5514545.
- Q2124139 wikiPageWikiLink Q7029056.
- Q2124139 wikiPageWikiLink Q76299.
- Q2124139 wikiPageWikiLink Q76359.
- Q2124139 wikiPageWikiLink Q853656.
- Q2124139 wikiPageWikiLink Q898896.
- Q2124139 wikiPageWikiLink Q902045.
- Q2124139 wikiPageWikiLink Q912313.
- Q2124139 wikiPageWikiLink Q9128.
- Q2124139 comment "RESOLFT, an acronym for REversible Saturable OpticaL Fluorescence Transitions, denotes a group of optical microscopy techniques with very high resolution. Using standard far field visible light optics a resolution far below the diffraction limit down to molecular scales can be obtained.With conventional microscopy techniques, it is not possible to distinguish features that are located at distances less than about half the wavelength used (i.e. about 200 nm for visible light).".
- Q2124139 label "RESOLFT".
- Q2124139 depiction RESOLFT_principle.jpg.