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- Q1952456 subject Q7164711.
- Q1952456 subject Q7400651.
- Q1952456 subject Q8249239.
- Q1952456 subject Q8579496.
- Q1952456 abstract "Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. The primer design for all primers pairs has to be optimized so that all primer pairs can work at the same annealing temperature during PCR. Multiplex-PCR was first described in 1988 as a method to detect deletions in the dystrophin gene. It has also been used with the steroid sulfatase gene. In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs.Multiplex-PCR consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple sequences at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. Alternatively, if amplicon sizes overlap, the different amplicons may be differentiated and visualised using primers that have been dyed with different colour fluorescent dyes. Commercial multiplexing kits for PCR are available and used by many forensic laboratories to amplify degraded DNA samples.".
- Q1952456 wikiPageExternalLink visual-omp.
- Q1952456 wikiPageExternalLink index.html.
- Q1952456 wikiPageExternalLink umb.php.
- Q1952456 wikiPageWikiLink Q130996.
- Q1952456 wikiPageWikiLink Q14864292.
- Q1952456 wikiPageWikiLink Q176996.
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- Q1952456 wikiPageWikiLink Q2844457.
- Q1952456 wikiPageWikiLink Q334828.
- Q1952456 wikiPageWikiLink Q4781505.
- Q1952456 wikiPageWikiLink Q48255.
- Q1952456 wikiPageWikiLink Q501128.
- Q1952456 wikiPageWikiLink Q574762.
- Q1952456 wikiPageWikiLink Q7164711.
- Q1952456 wikiPageWikiLink Q7400651.
- Q1952456 wikiPageWikiLink Q7430.
- Q1952456 wikiPageWikiLink Q8249239.
- Q1952456 wikiPageWikiLink Q8579496.
- Q1952456 comment "Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler.".
- Q1952456 label "Multiplex polymerase chain reaction".