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- Q1823823 subject Q10137627.
- Q1823823 abstract "The Liebermann–Burchard or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive.Lieberman–Burchard is a reagent used in a colourimetric test to detect cholesterol, which gives a deep green colour. This colour begins as a purplish, pink colour and progresses through to a light green then very dark green colour. The colour is due to the hydroxyl group (-OH) of cholesterol reacting with the reagents and increasing the conjugation of the un-saturation in the adjacent fused ring. Since this test uses acetic anhydride and sulfuric acid as reagents, caution must be exercised so as not to receive severe burns.Method : Dissolve one or two crystals of cholesterol in dry chloroform in a dry test tube. Add several drops of acetic anhydride and then 2 drops of conc.H2SO4 and mix carefully.After the reaction finished, the concentration of cholesterol can be measured using spectrophotometry.".
- Q1823823 wikiPageExternalLink 794.pdf.
- Q1823823 wikiPageWikiLink Q10137627.
- Q1823823 wikiPageWikiLink Q2356542.
- Q1823823 wikiPageWikiLink Q332084.
- Q1823823 wikiPageWikiLink Q407775.
- Q1823823 wikiPageWikiLink Q4118.
- Q1823823 wikiPageWikiLink Q43656.
- Q1823823 wikiPageWikiLink Q5149058.
- Q1823823 comment "The Liebermann–Burchard or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive.Lieberman–Burchard is a reagent used in a colourimetric test to detect cholesterol, which gives a deep green colour. This colour begins as a purplish, pink colour and progresses through to a light green then very dark green colour.".
- Q1823823 label "Liebermann–Burchard test".