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- Q17152027 subject Q8761843.
- Q17152027 abstract "Nucleoside phosphoramidites are derivatives of natural or synthetic nucleosides. They are used to synthesize oligonucleotides, relatively short fragments of nucleic acid and their analogs. Nucleoside phosphoramidites were first introduced in 1981 by Beaucage and Caruthers. In order to avoid undesired side reactions, reactive hydroxy and exocyclic amino groups present in natural or synthetic nucleosides are appropriately protected. As long as a nucleoside analog contains at least one hydroxy group, the use of the appropriate protecting strategy allows one to convert that to the respective phosphoramidite and to incorporate the latter into synthetic nucleic acids. In order to be incorporated in the middle of an oligonucleotide chain using phosphoramidite strategy, the nucleoside analog have to possess two hydroxy groups or, less often, a hydroxy group and another nucleophilic group (amino or mercapto). Examples include, but are not limited to, alternative nucleotides, LNA, morpholino, nucleosides modified at the 2'-position (OMe, protected NH2, F), nucleosides containing non-canonical bases (hypoxanthine and xanthine contained in natural nucleosides inosine and xanthosine, respectively, tricyclic bases such as G-clamp, etc.) or bases derivatized with a fluorescent group or a linker arm.".
- Q17152027 thumbnail Phosphoramidite1.png?width=300.
- Q17152027 wikiPageWikiLink Q101017.
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- Q17152027 wikiPageWikiLink Q671779.
- Q17152027 wikiPageWikiLink Q8761843.
- Q17152027 wikiPageWikiLink Q898525.
- Q17152027 wikiPageWikiLink Q903726.
- Q17152027 wikiPageWikiLink Q908955.
- Q17152027 wikiPageWikiLink Q926272.
- Q17152027 comment "Nucleoside phosphoramidites are derivatives of natural or synthetic nucleosides. They are used to synthesize oligonucleotides, relatively short fragments of nucleic acid and their analogs. Nucleoside phosphoramidites were first introduced in 1981 by Beaucage and Caruthers. In order to avoid undesired side reactions, reactive hydroxy and exocyclic amino groups present in natural or synthetic nucleosides are appropriately protected.".
- Q17152027 label "Nucleoside phosphoramidite".
- Q17152027 depiction Phosphoramidite1.png.