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- Q17141819 subject Q7216294.
- Q17141819 subject Q8421860.
- Q17141819 abstract "The lacUV5 promoter is a variant of the wild type Escherichia coli lac core promoter. It contains a 2 base pair mutation in the -10 hexamer region, compared to the lac promoter. By conforming closer to the sigma 70 consensus sequence, RNA Polymerase(RNAP) is recruited to the promoter more effectively, even in the absence of additional RNAP interactions via the C-terminal domain (CTD) of the alpha subunit of RNAP. Thus, transcription from lacUV5 will occur at higher rates than seen with Plac. Additionally, unlike Plac, PlacUV5 works independently of activator proteins or other cis regulatory elements (apart from the -10 and -35 promoter regions ). The lacUV5 promoter is commonly found on expression plasmids and is used when controllable but high levels of a product are desired.The lacUV5 promoter expression is regulated by the LacI repressor and can be induced with isopropyl thiogalactoside (IPTG). IPTG is an effective inducer of protein expression when used in the concentration range of 100μM to 1.5mM.The lacUV5 promoter is often found on plasmids used to express protein fusions for further study.".
- Q17141819 wikiPageWikiLink Q1093165.
- Q17141819 wikiPageWikiLink Q1295754.
- Q17141819 wikiPageWikiLink Q172778.
- Q17141819 wikiPageWikiLink Q174576.
- Q17141819 wikiPageWikiLink Q25419.
- Q17141819 wikiPageWikiLink Q272631.
- Q17141819 wikiPageWikiLink Q311359.
- Q17141819 wikiPageWikiLink Q4345509.
- Q17141819 wikiPageWikiLink Q7216294.
- Q17141819 wikiPageWikiLink Q8421860.
- Q17141819 comment "The lacUV5 promoter is a variant of the wild type Escherichia coli lac core promoter. It contains a 2 base pair mutation in the -10 hexamer region, compared to the lac promoter. By conforming closer to the sigma 70 consensus sequence, RNA Polymerase(RNAP) is recruited to the promoter more effectively, even in the absence of additional RNAP interactions via the C-terminal domain (CTD) of the alpha subunit of RNAP. Thus, transcription from lacUV5 will occur at higher rates than seen with Plac.".
- Q17141819 label "LacUV5".