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- Q1346346 subject Q13271623.
- Q1346346 subject Q7211577.
- Q1346346 subject Q8809956.
- Q1346346 abstract "Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective stain for Gram-negative bacteria. EMB contains dyes that are toxic for Gram positive bacteria and bile salt which is toxic for Gram negative bacteria other than coliforms. EMB is the selective and differential medium for coliforms. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1. A common application of this stain is in the preparation of EMB agar, a differential microbiological medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose (e.g., E. coli) and those that do not (e.g., Salmonella, Shigella). Organisms that ferment lactose display "nucleated colonies"—colonies with dark centers.This medium is important in medical laboratories by distinguishing pathogenic microbes in a short period of time.Rapid lactose fermentation produces acids, which lower the pH. This encourages dye absorption by the colonies, which are now colored purple-black.Lactose non-fermenters may increase the pH by deamination of proteins. This ensures that the dye is not absorbed. The colonies will be colorless. On EMB if E. coli is grown it will give a distinctive metallic green sheen (due to the metachromatic properties of the dyes, E. coli movement using flagella, and strong acid end-products of fermentation). Some species of Citrobacter and Enterobacter will also react this way to EMB.This medium has been specifically designed to discourage the growth of gram positive bacteria.EMB contains the following ingredients: peptone, lactose, dipotassium phosphate, eosin Y (dye), methylene blue (dye), and agar.There are also EMB agars that do not contain lactose.".
- Q1346346 thumbnail Coli_levine.JPG?width=300.
- Q1346346 wikiPageExternalLink 17.
- Q1346346 wikiPageExternalLink eosin_methylene_blue_agar.php.
- Q1346346 wikiPageExternalLink Eosin_Methylene_Blue_Agar_Modified.pdf.
- Q1346346 wikiPageExternalLink details.asp?id=2553&Lang=.
- Q1346346 wikiPageExternalLink product_info.php?products_id=10038.
- Q1346346 wikiPageExternalLink 7103_PI.pdf.
- Q1346346 wikiPageWikiLink Q127900.
- Q1346346 wikiPageWikiLink Q13271623.
- Q1346346 wikiPageWikiLink Q177998.
- Q1346346 wikiPageWikiLink Q2332446.
- Q1346346 wikiPageWikiLink Q25419.
- Q1346346 wikiPageWikiLink Q403721.
- Q1346346 wikiPageWikiLink Q422134.
- Q1346346 wikiPageWikiLink Q422637.
- Q1346346 wikiPageWikiLink Q575920.
- Q1346346 wikiPageWikiLink Q632006.
- Q1346346 wikiPageWikiLink Q7211577.
- Q1346346 wikiPageWikiLink Q857288.
- Q1346346 wikiPageWikiLink Q8809956.
- Q1346346 comment "Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective stain for Gram-negative bacteria. EMB contains dyes that are toxic for Gram positive bacteria and bile salt which is toxic for Gram negative bacteria other than coliforms. EMB is the selective and differential medium for coliforms. It is a blend of two stains, eosin and methylene blue in the ratio of 6:1.".
- Q1346346 label "Eosin methylene blue".
- Q1346346 depiction Coli_levine.JPG.