Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Thin-layer_chromatography> ?p ?o }
- Thin-layer_chromatography abstract "Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.After the sample has been applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved. The mobile phase has different properties than the stationary phase. For example, with silica gel, a very polar substance, non-polar mobile phases such as heptane are used. The mobile phase may be a mixture, allowing chemists to fine-tune the bulk properties of the mobile phase.After the experiment, the spots are visualized. Often this can be done simply by projecting ultraviolet light onto the sheet; the sheets are treated with a phosphor, and dark spots appear on the sheet where compounds absorb the light impinging on a certain area. Chemical processes can also be used to visualize spots; anisaldehyde, for example, forms colored adducts with many compounds, and sulfuric acid will char most organic compounds, leaving a dark spot on the sheet.To quantify the results, the distance traveled by the substance being considered is divided by the total distance traveled by the mobile phase. (The mobile phase must not be allowed to reach the end of the stationary phase.) This ratio is called the retention factor or Rf. In general,a substance whose structure resembles the stationary phase will have low Rf, while one that has a similar structure to the mobile phase will have high retention factor. Retention factors are characteristic, but will change depending on the exact condition of the mobile and stationary phase. For this reason, chemists usually apply a sample of a known compound to the sheet before running the experiment.Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance. Specific examples of these applications include: analyzing ceramides and fatty acids, detection of pesticides or insecticides in food and water, analyzing the dye composition of fibers in forensics, assaying the radiochemical purity of radiopharmaceuticals, or identification of medicinal plants and their constituents A number of enhancements can be made to the original method to automate the different steps, to increase the resolution achieved with TLC and to allow more accurate quantitative analysis. This method is referred to as HPTLC, or \"high-performance TLC\". HPTLC typically uses thinner layers of stationary phase and smaller sample volumes, thus reducing the loss of resolution due to diffusion.".
- Thin-layer_chromatography thumbnail TLC_black_ink.jpg?width=300.
- Thin-layer_chromatography wikiPageID "2152225".
- Thin-layer_chromatography wikiPageLength "17792".
- Thin-layer_chromatography wikiPageOutDegree "92".
- Thin-layer_chromatography wikiPageRevisionID "702477989".
- Thin-layer_chromatography wikiPageWikiLink 2-Naphthol.
- Thin-layer_chromatography wikiPageWikiLink 4-Anisaldehyde.
- Thin-layer_chromatography wikiPageWikiLink Acetic_acid.
- Thin-layer_chromatography wikiPageWikiLink Acetone.
- Thin-layer_chromatography wikiPageWikiLink Acetonitrile.
- Thin-layer_chromatography wikiPageWikiLink Adsorption.
- Thin-layer_chromatography wikiPageWikiLink Agarose_gel_electrophoresis.
- Thin-layer_chromatography wikiPageWikiLink Aluminium_oxide.
- Thin-layer_chromatography wikiPageWikiLink Analyte.
- Thin-layer_chromatography wikiPageWikiLink BINAP.
- Thin-layer_chromatography wikiPageWikiLink Benzene.
- Thin-layer_chromatography wikiPageWikiLink Black_light.
- Thin-layer_chromatography wikiPageWikiLink Bromine.
- Thin-layer_chromatography wikiPageWikiLink Calcium_sulfate.
- Thin-layer_chromatography wikiPageWikiLink Capillary_action.
- Thin-layer_chromatography wikiPageWikiLink Carbon_tetrachloride.
- Thin-layer_chromatography wikiPageWikiLink Carotene.
- Thin-layer_chromatography wikiPageWikiLink Category:Chromatography.
- Thin-layer_chromatography wikiPageWikiLink Category:Laboratory_techniques.
- Thin-layer_chromatography wikiPageWikiLink Cellulose.
- Thin-layer_chromatography wikiPageWikiLink Ceramide.
- Thin-layer_chromatography wikiPageWikiLink Chemical_compound.
- Thin-layer_chromatography wikiPageWikiLink Chemical_reaction.
- Thin-layer_chromatography wikiPageWikiLink Chlorophyll.
- Thin-layer_chromatography wikiPageWikiLink Chromatography.
- Thin-layer_chromatography wikiPageWikiLink Column_chromatography.
- Thin-layer_chromatography wikiPageWikiLink Dichloromethane.
- Thin-layer_chromatography wikiPageWikiLink Diethyl_ether.
- Thin-layer_chromatography wikiPageWikiLink Diffusion.
- Thin-layer_chromatography wikiPageWikiLink Diisobutylaluminium_hydride.
- Thin-layer_chromatography wikiPageWikiLink Elution.
- Thin-layer_chromatography wikiPageWikiLink Ethyl_acetate.
- Thin-layer_chromatography wikiPageWikiLink Far-Eastern_blotting.
- Thin-layer_chromatography wikiPageWikiLink Fatty_acid.
- Thin-layer_chromatography wikiPageWikiLink Fluorescence.
- Thin-layer_chromatography wikiPageWikiLink Fluorocarbon.
- Thin-layer_chromatography wikiPageWikiLink Forensic_science.
- Thin-layer_chromatography wikiPageWikiLink Formic_acid.
- Thin-layer_chromatography wikiPageWikiLink Glass.
- Thin-layer_chromatography wikiPageWikiLink Heptane.
- Thin-layer_chromatography wikiPageWikiLink Hexane.
- Thin-layer_chromatography wikiPageWikiLink High_performance_thin_layer_chromatography.
- Thin-layer_chromatography wikiPageWikiLink Inert.
- Thin-layer_chromatography wikiPageWikiLink Insecticide.
- Thin-layer_chromatography wikiPageWikiLink Iodine.
- Thin-layer_chromatography wikiPageWikiLink Isopropyl_alcohol.
- Thin-layer_chromatography wikiPageWikiLink Lutein.
- Thin-layer_chromatography wikiPageWikiLink Manganese.
- Thin-layer_chromatography wikiPageWikiLink Mass_spectrometry.
- Thin-layer_chromatography wikiPageWikiLink Medicinal_plants.
- Thin-layer_chromatography wikiPageWikiLink Medicinal_radiocompounds.
- Thin-layer_chromatography wikiPageWikiLink Methanol.
- Thin-layer_chromatography wikiPageWikiLink N-Butanol.
- Thin-layer_chromatography wikiPageWikiLink Organic_chemistry.
- Thin-layer_chromatography wikiPageWikiLink Organic_reaction.
- Thin-layer_chromatography wikiPageWikiLink Paper_chromatography.
- Thin-layer_chromatography wikiPageWikiLink Pentane.
- Thin-layer_chromatography wikiPageWikiLink Pesticide.
- Thin-layer_chromatography wikiPageWikiLink Phosphor.
- Thin-layer_chromatography wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Thin-layer_chromatography wikiPageWikiLink Polyvinylidene_fluoride.
- Thin-layer_chromatography wikiPageWikiLink Potassium_permanganate.
- Thin-layer_chromatography wikiPageWikiLink Radioactive_decay.
- Thin-layer_chromatography wikiPageWikiLink Radiochemical_purity.
- Thin-layer_chromatography wikiPageWikiLink Reagent.
- Thin-layer_chromatography wikiPageWikiLink Retardation_factor.
- Thin-layer_chromatography wikiPageWikiLink Reversed-phase_chromatography.
- Thin-layer_chromatography wikiPageWikiLink Silica_gel.
- Thin-layer_chromatography wikiPageWikiLink Solution.
- Thin-layer_chromatography wikiPageWikiLink Solvent.
- Thin-layer_chromatography wikiPageWikiLink Spectrophotometry.
- Thin-layer_chromatography wikiPageWikiLink Spinach.
- Thin-layer_chromatography wikiPageWikiLink Sulfuric_acid.
- Thin-layer_chromatography wikiPageWikiLink Toluene.
- Thin-layer_chromatography wikiPageWikiLink Triethylamine.
- Thin-layer_chromatography wikiPageWikiLink Ultraviolet.
- Thin-layer_chromatography wikiPageWikiLink Vacuum_chamber.
- Thin-layer_chromatography wikiPageWikiLink Water.
- Thin-layer_chromatography wikiPageWikiLink Willemite.
- Thin-layer_chromatography wikiPageWikiLink Zinc_sulfide.
- Thin-layer_chromatography wikiPageWikiLink File:Chromatography00.jpg.
- Thin-layer_chromatography wikiPageWikiLink File:TLC-Essential-Oils.jpg.
- Thin-layer_chromatography wikiPageWikiLink File:TLC_-_isomers.jpg.
- Thin-layer_chromatography wikiPageWikiLink File:Tlc_sequence.svg.
- Thin-layer_chromatography wikiPageWikiLinkText "TLC".
- Thin-layer_chromatography wikiPageWikiLinkText "Thin-layer chromatography".
- Thin-layer_chromatography wikiPageWikiLinkText "thin layer chromatograms".
- Thin-layer_chromatography wikiPageWikiLinkText "thin-layer chromatography".
- Thin-layer_chromatography acronym "TLC".
- Thin-layer_chromatography caption "Separation of black ink on a TLC plate".
- Thin-layer_chromatography classification Chromatography.
- Thin-layer_chromatography name "Thin-layer chromatography".
- Thin-layer_chromatography related Agarose_gel_electrophoresis.
- Thin-layer_chromatography related Polyacrylamide_gel_electrophoresis.