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- Tandem_affinity_purification abstract "Tandem affinity purification (TAP) is a purification technique for studying protein-protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end.In the original version of the technique, the protein of interest with the TAP tag first binds to beads coated with IgG, the TAP tag is then broken apart by an enzyme, and finally a different part of the TAP tag binds reversibly to beads of a different type. After the protein of interest has been washed through two affinity columns, it can be examined for binding partners.The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.Many other tag combinations have been proposed since the TAP principle was first published.".
- Tandem_affinity_purification thumbnail Taptag_simple.png?width=300.
- Tandem_affinity_purification wikiPageID "9931632".
- Tandem_affinity_purification wikiPageLength "9963".
- Tandem_affinity_purification wikiPageOutDegree "29".
- Tandem_affinity_purification wikiPageRevisionID "700822988".
- Tandem_affinity_purification wikiPageWikiLink Bond_cleavage.
- Tandem_affinity_purification wikiPageWikiLink C-terminus.
- Tandem_affinity_purification wikiPageWikiLink Calmodulin.
- Tandem_affinity_purification wikiPageWikiLink Category:Protein–protein_interaction_assays.
- Tandem_affinity_purification wikiPageWikiLink EGTA_(chemical).
- Tandem_affinity_purification wikiPageWikiLink European_Molecular_Biology_Laboratory.
- Tandem_affinity_purification wikiPageWikiLink Fusion_protein.
- Tandem_affinity_purification wikiPageWikiLink Immunoglobulin_G.
- Tandem_affinity_purification wikiPageWikiLink In_vivo.
- Tandem_affinity_purification wikiPageWikiLink Ligand_(biochemistry).
- Tandem_affinity_purification wikiPageWikiLink Mass_spectrometry.
- Tandem_affinity_purification wikiPageWikiLink N-terminus.
- Tandem_affinity_purification wikiPageWikiLink Photo-reactive_amino_acid_analog.
- Tandem_affinity_purification wikiPageWikiLink Plasmid.
- Tandem_affinity_purification wikiPageWikiLink Polyacrylamide_gel_electrophoresis.
- Tandem_affinity_purification wikiPageWikiLink Protease.
- Tandem_affinity_purification wikiPageWikiLink Protein_A.
- Tandem_affinity_purification wikiPageWikiLink Protein–protein_interaction.
- Tandem_affinity_purification wikiPageWikiLink Proteomics.
- Tandem_affinity_purification wikiPageWikiLink TEV_protease.
- Tandem_affinity_purification wikiPageWikiLink Transient_protein_interactions.
- Tandem_affinity_purification wikiPageWikiLink Translation.
- Tandem_affinity_purification wikiPageWikiLink Two-hybrid_screening.
- Tandem_affinity_purification wikiPageWikiLink Wikt:specificity.
- Tandem_affinity_purification wikiPageWikiLink Yeast.
- Tandem_affinity_purification wikiPageWikiLink File:Taptag_simple.png.
- Tandem_affinity_purification wikiPageWikiLinkText "TAP tag library".
- Tandem_affinity_purification wikiPageWikiLinkText "Tandem affinity purification".
- Tandem_affinity_purification wikiPageWikiLinkText "affinity purification".
- Tandem_affinity_purification wikiPageWikiLinkText "tandem affinity purification".
- Tandem_affinity_purification wikiPageUsesTemplate Template:Cn.
- Tandem_affinity_purification subject Category:Protein–protein_interaction_assays.
- Tandem_affinity_purification hypernym Technique.
- Tandem_affinity_purification type TopicalConcept.
- Tandem_affinity_purification type Method.
- Tandem_affinity_purification type Technique.
- Tandem_affinity_purification comment "Tandem affinity purification (TAP) is a purification technique for studying protein-protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end.In the original version of the technique, the protein of interest with the TAP tag first binds to beads coated with IgG, the TAP tag is then broken apart by an enzyme, and finally a different part of the TAP tag binds reversibly to beads of a different type.".
- Tandem_affinity_purification label "Tandem affinity purification".
- Tandem_affinity_purification sameAs Q893051.
- Tandem_affinity_purification sameAs Tandem_Affinity_Purification.
- Tandem_affinity_purification sameAs Méthode_TAP.
- Tandem_affinity_purification sameAs m.02pxcdv.
- Tandem_affinity_purification sameAs Q893051.
- Tandem_affinity_purification wasDerivedFrom Tandem_affinity_purification?oldid=700822988.
- Tandem_affinity_purification depiction Taptag_simple.png.
- Tandem_affinity_purification isPrimaryTopicOf Tandem_affinity_purification.