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- RoGFP abstract "The reduction-oxidation sensitive green fluorescent protein (roGFP) is a redox sensitive biosensor. Two cysteines were introduced into the beta barrel structure of the GFP. The oxidation state (reduced dithiol or the oxidized disulfide) of the engineered thiols determines the fluorescence properties of the sensor. Originally, different roGFP versions were presented to allow the in vivo imaging of reducing compartments such as the cytosol (roGFP2). The cysteines introduced at the amino acid positions 147 and 204 produced the most robust results. roGFP2 preferentially interacts with glutaredoxins and therefore reports the cellular glutathione redox potential. The specificity of roGFP2 for glutathione is further increased by linking it to the human glutaredoxin 1 (Grx1).By expressing the Grx1-roGFP fusion sensors in the organism of interest and/or targeting the protein to a cellular compartment, it is possible to measure the glutathione redox potential in a specific cellular compartment in real-time and therefore provides major advantages compared to other invasive static methods e.g. HPLC.In addition, roGFPs are used to investigate the topology of ER proteins, or to analyze the ROS production capacity of chemicals.".
- RoGFP wikiPageID "22064427".
- RoGFP wikiPageLength "4108".
- RoGFP wikiPageOutDegree "24".
- RoGFP wikiPageRevisionID "704878430".
- RoGFP wikiPageWikiLink Amino_acid.
- RoGFP wikiPageWikiLink Biosensor.
- RoGFP wikiPageWikiLink Category:Biochemistry_methods.
- RoGFP wikiPageWikiLink Category:Cell_imaging.
- RoGFP wikiPageWikiLink Category:Fluorescent_dyes.
- RoGFP wikiPageWikiLink Chemical_substance.
- RoGFP wikiPageWikiLink Cysteine.
- RoGFP wikiPageWikiLink Cytosol.
- RoGFP wikiPageWikiLink Disulfide.
- RoGFP wikiPageWikiLink Endoplasmic_reticulum.
- RoGFP wikiPageWikiLink Fluorescence.
- RoGFP wikiPageWikiLink Glutaredoxin.
- RoGFP wikiPageWikiLink Glutathione.
- RoGFP wikiPageWikiLink Green_fluorescent_protein.
- RoGFP wikiPageWikiLink High-performance_liquid_chromatography.
- RoGFP wikiPageWikiLink Protein.
- RoGFP wikiPageWikiLink Reactive_oxygen_species.
- RoGFP wikiPageWikiLink Redox.
- RoGFP wikiPageWikiLink Reduction_potential.
- RoGFP wikiPageWikiLink Synapto-pHluorin.
- RoGFP wikiPageWikiLinkText "roGFP".
- RoGFP wikiPageUsesTemplate Template:Reflist.
- RoGFP subject Category:Biochemistry_methods.
- RoGFP subject Category:Cell_imaging.
- RoGFP subject Category:Fluorescent_dyes.
- RoGFP hypernym Biosensor.
- RoGFP type Method.
- RoGFP type Technique.
- RoGFP comment "The reduction-oxidation sensitive green fluorescent protein (roGFP) is a redox sensitive biosensor. Two cysteines were introduced into the beta barrel structure of the GFP. The oxidation state (reduced dithiol or the oxidized disulfide) of the engineered thiols determines the fluorescence properties of the sensor. Originally, different roGFP versions were presented to allow the in vivo imaging of reducing compartments such as the cytosol (roGFP2).".
- RoGFP label "RoGFP".
- RoGFP sameAs Q7339213.
- RoGFP sameAs RoGFP.
- RoGFP sameAs m.05n_20v.
- RoGFP sameAs Q7339213.
- RoGFP wasDerivedFrom RoGFP?oldid=704878430.
- RoGFP isPrimaryTopicOf RoGFP.