Matches in DBpedia 2016-04 for { <http://dbpedia.org/resource/Polymerase_chain_reaction> ?p ?o }
- Polymerase_chain_reaction abstract "The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and DNA paternity testing); and the detection and diagnosis of infectious diseases. In 1993, Mullis was awarded the Nobel Prize in Chemistry along with Michael Smith for his work on PCR.The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase, which the method is named after, are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations.Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase (an enzyme originally isolated from the bacterium Thermus aquaticus). This DNA polymerase enzymatically assembles a new DNA strand from DNA building-blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis. The vast majority of PCR methods use thermal cycling, i.e., alternately heating and cooling the PCR sample through a defined series of temperature steps.In the first step, the two strands of the DNA double helix are physically separated at a high temperature in a process called DNA melting. In the second step, the temperature is lowered and the two DNA strands become templates for DNA polymerase to selectively amplify the target DNA. The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions.".
- Polymerase_chain_reaction thumbnail PCR_tubes.png?width=300.
- Polymerase_chain_reaction wikiPageExternalLink genewarrior.com.
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- Polymerase_chain_reaction wikiPageExternalLink openpcr.org.
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- Polymerase_chain_reaction wikiPageExternalLink pcr_buying_guide.aspx.
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- Polymerase_chain_reaction wikiPageWikiLink 16S_rRNA.
- Polymerase_chain_reaction wikiPageWikiLink Agarose_gel_electrophoresis.
- Polymerase_chain_reaction wikiPageWikiLink Allele.
- Polymerase_chain_reaction wikiPageWikiLink Allele-specific_PCR.
- Polymerase_chain_reaction wikiPageWikiLink Amplicon.
- Polymerase_chain_reaction wikiPageWikiLink Anaerobic_organism.
- Polymerase_chain_reaction wikiPageWikiLink Ancient_DNA.
- Polymerase_chain_reaction wikiPageWikiLink Antibody.
- Polymerase_chain_reaction wikiPageWikiLink Arithmetic.
- Polymerase_chain_reaction wikiPageWikiLink Asymmetric_PCR.
- Polymerase_chain_reaction wikiPageWikiLink Bacteriophage.
- Polymerase_chain_reaction wikiPageWikiLink Base_pair.
- Polymerase_chain_reaction wikiPageWikiLink Biotechnology.
- Polymerase_chain_reaction wikiPageWikiLink Bisulfite_sequencing.
- Polymerase_chain_reaction wikiPageWikiLink Bivalent.
- Polymerase_chain_reaction wikiPageWikiLink Black_Death.
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- Polymerase_chain_reaction wikiPageWikiLink California_State_Route_1.
- Polymerase_chain_reaction wikiPageWikiLink Category:1983_introductions.
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- Polymerase_chain_reaction wikiPageWikiLink Category:Amplifiers.
- Polymerase_chain_reaction wikiPageWikiLink Category:Biotechnology.
- Polymerase_chain_reaction wikiPageWikiLink Category:DNA_profiling_techniques.
- Polymerase_chain_reaction wikiPageWikiLink Category:Hoffmann-La_Roche.
- Polymerase_chain_reaction wikiPageWikiLink Category:Laboratory_techniques.
- Polymerase_chain_reaction wikiPageWikiLink Category:Molecular_biology.
- Polymerase_chain_reaction wikiPageWikiLink Category:Molecular_biology_techniques.
- Polymerase_chain_reaction wikiPageWikiLink Category:Polymerase_chain_reaction.
- Polymerase_chain_reaction wikiPageWikiLink Cetus_Corporation.
- Polymerase_chain_reaction wikiPageWikiLink Chain_reaction.
- Polymerase_chain_reaction wikiPageWikiLink Cold_Spring_Harbor_Laboratory.
- Polymerase_chain_reaction wikiPageWikiLink Complementarity_(molecular_biology).
- Polymerase_chain_reaction wikiPageWikiLink Complementary_DNA.
- Polymerase_chain_reaction wikiPageWikiLink Cosmid.
- Polymerase_chain_reaction wikiPageWikiLink DNA.
- Polymerase_chain_reaction wikiPageWikiLink DNA_footprinting.
- Polymerase_chain_reaction wikiPageWikiLink DNA_paternity_testing.
- Polymerase_chain_reaction wikiPageWikiLink DNA_polymerase.
- Polymerase_chain_reaction wikiPageWikiLink DNA_profiling.
- Polymerase_chain_reaction wikiPageWikiLink DNA_replication.
- Polymerase_chain_reaction wikiPageWikiLink DNA_sequencing.
- Polymerase_chain_reaction wikiPageWikiLink DNA–DNA_hybridization.
- Polymerase_chain_reaction wikiPageWikiLink Denaturation_(biochemistry).
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- Polymerase_chain_reaction wikiPageWikiLink Directionality_(molecular_biology).
- Polymerase_chain_reaction wikiPageWikiLink DuPont.
- Polymerase_chain_reaction wikiPageWikiLink Emeryville,_California.
- Polymerase_chain_reaction wikiPageWikiLink Enzyme.
- Polymerase_chain_reaction wikiPageWikiLink Escherichia_coli.
- Polymerase_chain_reaction wikiPageWikiLink Exon.
- Polymerase_chain_reaction wikiPageWikiLink Exponential_growth.
- Polymerase_chain_reaction wikiPageWikiLink Fluorophore.
- Polymerase_chain_reaction wikiPageWikiLink Forensic_science.
- Polymerase_chain_reaction wikiPageWikiLink Formamide.
- Polymerase_chain_reaction wikiPageWikiLink Gel_electrophoresis.
- Polymerase_chain_reaction wikiPageWikiLink Gene.
- Polymerase_chain_reaction wikiPageWikiLink Gene_expression.
- Polymerase_chain_reaction wikiPageWikiLink Gene_expression_profiling.
- Polymerase_chain_reaction wikiPageWikiLink Genetic_disorder.
- Polymerase_chain_reaction wikiPageWikiLink Genetic_engineering.
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- Polymerase_chain_reaction wikiPageWikiLink Genomics.
- Polymerase_chain_reaction wikiPageWikiLink Har_Gobind_Khorana.
- Polymerase_chain_reaction wikiPageWikiLink Helicase.
- Polymerase_chain_reaction wikiPageWikiLink Helicase-dependent_amplification.
- Polymerase_chain_reaction wikiPageWikiLink Hoffmann-La_Roche.
- Polymerase_chain_reaction wikiPageWikiLink Hot_start_PCR.
- Polymerase_chain_reaction wikiPageWikiLink Hybridization_probe.
- Polymerase_chain_reaction wikiPageWikiLink Hydroxyl.
- Polymerase_chain_reaction wikiPageWikiLink In_silico_PCR.
- Polymerase_chain_reaction wikiPageWikiLink In_vitro.
- Polymerase_chain_reaction wikiPageWikiLink Infection.
- Polymerase_chain_reaction wikiPageWikiLink Intersequence-specific_PCR.
- Polymerase_chain_reaction wikiPageWikiLink Intron.
- Polymerase_chain_reaction wikiPageWikiLink Inverse_polymerase_chain_reaction.
- Polymerase_chain_reaction wikiPageWikiLink Ion.
- Polymerase_chain_reaction wikiPageWikiLink Journal_of_Molecular_Biology.
- Polymerase_chain_reaction wikiPageWikiLink Kary_Mullis.
- Polymerase_chain_reaction wikiPageWikiLink Kjell_Kleppe.
- Polymerase_chain_reaction wikiPageWikiLink Leukemia.
- Polymerase_chain_reaction wikiPageWikiLink Ligation-mediated_PCR.